t take it anymore t tan total acid numb t test t tetn are good t the damned telephon t the death of a idea t the end t the one you should t the passion t the suit t the things you do y t think less climb mo t through the mud t transformer t tribute to gladys t trust hi...
Step 1—Obtaining the mutant insert by error-prone PCR and the control insert The DsRed2 gene was isolated using the plasmid pDsRed2 (Clontech, Cat. No. 632404, UniProt Q9U6Y8) as a template (Fig.1A). Error-prone PCR of the DsRed2 gene was performed using the GeneMorph® II Rando...
In this study, we developed a droplet-based, high-throughput, and high-sensitivity single-microbe RNA-seq method, named smRandom-seq, with the pipeline of bacteria preparation, reactions in situ, droplet barcoding, and library preparation. We provided sufficient and systematic evidence to prove the...
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Thetet(M) PCR mixture (50 µL final volume) contained 1X PCR buffer (Promega), 3 mM MgCl2, 0.2 mM dNTPs, 1 μM of each primers (IntMtet1 and IntMtet2), 1 U of HotStart G2 DNA polymerase (Promega), and 5 μL of DNA template. Amplification was performed as descr...
All the MR brain images were aligned along the line that passes through the anterior and posterior commissures of the brain, corrected for their bias field, and finally spatially normalized to the MNI152 template space using affine transformation35. ...