Food and Drug Administration (USFDA) approved the first liquid biopsy, Cobas EGFR. The test uses plasma specimens to identify mutations in the epidermal growth factor receptor (EGFR) gene. Other companion diagnostic tests such as Mutation Test v2 have also been approved and identify patients with...
We compared two advanced random test generators, a Feedback-Directed Random Test Generator (FDR) and a Feedback-Controlled Random Test Generator (FCR), for an automated software testing tool in Python 2.x, the Template Scripting Testing Language (TSTL).An FDR generates test inputs incrementally...
The crosslinked proteinosomes were phase transferred into water, and the addition of biotin-functionalized or dsDNA strands led to DNA template accumulation and stable retention up to 200 base pairs (bp) (ref. 37). In previous work37, we have characterized the proteinosome membrane and ...
template-switching strategy for the second strand cDNA synthesis. The template-switching strategy could be affected by the complex structure of RNAs and fail to capture interrupted cDNAs42. Thus, we adapted a dA tailing strategy for efficient second-strand cDNA synthesis, as non-template dA can ...
template-switching strategy for the second strand cDNA synthesis. The template-switching strategy could be affected by the complex structure of RNAs and fail to capture interrupted cDNAs42. Thus, we adapted a dA tailing strategy for efficient second-strand cDNA synthesis, as non-template dA can ...
Template-based protein structure prediction in CASP11 and retrospect of I-TASSER in the last decade. Proteins Struct Funct Bioinformatics. 2016;84(S1):233–46. 30. Katoh E, Hatta T, Shindo H, et al. High precision NMR structure of YhhP, a novel Escherichia coli protein implicated in ...
Ray et al. evaluated the regional alterations in the ADC of cortical gray and white matter and subcortical structures, which are known to be involved in MCI, stating that ADC from gray and white matter of different brain regions could be analyzed by applying an automated template-masking method...
“overhangs” to template molecules. These overhangs contain useful components, such as inline indices, UMIs, and read offsets, which we discuss in detail below, as well as a “universal” priming site for the standard Illumina primers used in the second PCR. The second PCR is typically ...
The PCR for CPEC was carried out using the TAKARA LATaq enzyme(Clontech Cat. No. RR002A), following the conditions: 94 °C/2 min, 30 cycles of 94 °C/15 s, 63 °C/30 s, 68 °C/4 min and 1 final cycle 72 °C/5 min. The template DNA for the CPEC reaction was the doubl...
Briefly, Poly(A)+ mRNA from extracted total RNA were converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples were then pooled, amplified (10 PCR cycles...