t take it anymore t tan total acid numb t test t tetn are good t the damned telephon t the death of a idea t the end t the one you should t the passion t the suit t the things you do y t think less climb mo t through the mud t transformer t tribute to gladys t trust hi...
Step 1—Obtaining the mutant insert by error-prone PCR and the control insert The DsRed2 gene was isolated using the plasmid pDsRed2 (Clontech, Cat. No. 632404, UniProt Q9U6Y8) as a template (Fig.1A). Error-prone PCR of the DsRed2 gene was performed using the GeneMorph® II Rando...
In this study, we developed a droplet-based, high-throughput, and high-sensitivity single-microbe RNA-seq method, named smRandom-seq, with the pipeline of bacteria preparation, reactions in situ, droplet barcoding, and library preparation. We provided sufficient and systematic evidence to prove the...
Generalizing these ideas and performing them efficiently leads to the max-product algorithm, a general template for performing such computations exactly in arbitrary tree-structured graphs. The max-product or max-sum algorithm The max-product algorithm can be used to find the most probable explanation...
A comprehensive assessment of sequence-based and template-based methods for protein contact prediction. Bioinformatics. 2008;24:924–31. Luttrell et al. BMC Bioinformatics 2019, 20(Suppl 2):100 15. Shao Y, Bystroff C. Predicting interresidue contacts using templates and pathways. Proteins. 2003...
In addition to the opinions in the template letter, here is a template letter which I have copied from the Internet (sorry, I do not have the source for credit) and have added more reasons for Sheung Shui residents for the last consultation conducted in Sept 2012: To whom it may concern...
template. It is preferable to commence the first action in each action block after an indicated incubation period, if any, has passed subsequent to completion of the previous action block. Along these lines, it is also preferable to separate each pair of action blocks in an assay template by...
More Template Letter In addition to the opinions in the template letter, here is a template letter which I have copied from the Internet (sorry, I do not have the source for credit) and have added more reasons for Sheung Shui residents for the last consultation conducted in Sept 2012: To...
Thetet(M) PCR mixture (50 µL final volume) contained 1X PCR buffer (Promega), 3 mM MgCl2, 0.2 mM dNTPs, 1 μM of each primers (IntMtet1 and IntMtet2), 1 U of HotStart G2 DNA polymerase (Promega), and 5 μL of DNA template. Amplification was performed as descr...
Briefly, Poly(A)+ mRNA from extracted total RNA were converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples were then pooled, amplified (10 PCR cycles...