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BP terms underrepresented among 2ROs (Additional file 5, Table S3_bp) were dominated by basic cellular functions strongly conserved throughout Eukaryota, such as translation, DNA repair, RNA splicing, DNA replication, protein folding, DNA recombination, cellular respiration, mRNA transport or ubiquitin...
Full size image FANCI affects R-loop distribution resulting in tumor progression in LUAD To plot the R-loop landscape of LUAD cells, we conducted DRIP-seq using S9.6, an anti-DNA/RNA hybrid antibody. In LUAD cell lines (PC-9), genome-wide R-loop signal profile showed that the number...
DNA was retrieved on glass filters (LKB, Bromma, Sweden) and radioactivity was counted by a scintillation counter (Betaplate 1205-012, LKB). Northern blotting Total RNA was extracted from CL-1 cells using ISOGEN (Nippon Gene, Tokyo, Japan). One microgram of total RNA was separated on 1% ...
After injection, CISD KO flies were identified by PCR and subsequent DNA sequencing. Measurement of calcium levels in Drosophila ERGCaMP6-210 was expressed in the muscle by mef2-GAL4 and UAS-ERGCaMP6-210 for ER calcium measurement in Drosophila larval muscle. GCaMP5G was also expressed by...
Hybridization with 32P-la-beled 1.4-kb Xho fragment of cDNA pl3527 was performed overnight in 50% formamide, 3x SCC, 0.1% sodium dodecyl sulfate (SDS), 10X Denhardt's solution, with 50 μg/mL denatured Salmon sperm DNA at 42°C. Filters were washed in 0.3X SCC, 0.1% SDS at 42°...
Full size image Chemical characterization of compound 5 The purified active (estrogenic) sample, compound 5 (2.2 mg), was identified by NMR, optical rotation, and circular dichroism as 2,3-cis-(2R,3R)-(−)-epiafzelechin-3-O-p-coumarate (rhodoeosein, [Figure 3]). Table 1 shows the ...
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