the genome quality still needs improvements to precisely identify genes and variations underlying important selected traits. For example, the gene numbers of TPS family in these studies varied, especially those in functionally important TPS-b clade. Besides, the currentC. camphoragenome studies mainly ...
sex-specific genes are derived from — and therefore homologous to — genes that might also be present in the other sex, and accumulation of highly repetitive DNA sequences due to non-recombination hinders the analysis
Welsh J, Ralph D, McClelland M: DNA and RNA fingerprinting using arbitrarily primed PCR. PCR Strategies. Edited by: Innis MA, Gelfand DH, Sninsky JJ. 1995, San Diego: Academic Press Google Scholar Kostia S, Palo J, Varvio SL: DNA sequences of RAPD fragments in artiodactyls. Genome....
Mobile element insertion is one type of structure variation that is ideal for this purpose. Mobile elements (MEs) are segments of DNA that contribute to at least 50% of the human genome [19, 20]. MEs can move around within the genome and create new insertions. As a result, thousands of...
Cytidine deaminase is an enzyme that converts C-to-U through a hydrolytic deamination reaction (Fig.3). It is found in both prokaryotic and eukaryotic systems and has the ability to deaminate cytidine in both DNA and RNA. APOBECs, a class of enzymes that perform cytidine deamination, include...
Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing Yuebo Zhang, Longchao Zhang, Jingwei Yue, Xia Wei, Ligang Wang, Xin Liu, Hongmei Gao, Xinhua Hou, Fuping Zhao, Hua Yan & Lixian Wang Journal of Animal Science...
The quality of DNA was assessed through electrophoresis in a 1% agarose gel and staining with SYBR-safe DNA gel stain (Invitrogen). PCR amplification and sequencing of L. macaronius mtDNA PCR amplification was performed using a mix of insect universal primers and primers specifically designed ...
can function as a DNA and RNA demethylase [22,23] but also exhibits abasic (apyrimidinic/apurinic) lyase activity [24]. Several studies demonstrated that ALKBH1 has the demethylase activity of 6mA on single-stranded DNA [5,7,13,14]. More recent studies indicated that ALKBH1 functions to...
Antarctica and its unique biodiversity are increasingly at risk from the effects of global climate change and other human influences. A significant recent element underpinning strategies for Antarctic conservation has been the development of a system of
During the transfer of DNA fragments from chloroplasts to the mitogenome, it is common for some fragments to carry PCGs. However, these transferred genes often become nonfunctional pseudogenes [29]. In our study, we identified a set of intact plastid genes associated with photosynthesis (psbJ, ...