对于 Illumina 的QuantSeq3' mRNA-Seq 文库制备试剂盒中的barcodes允许在 Illumina flow cell每条通道上测序的样本多达96个。对于Ion Torrent的QuantSeq3' mRNA-Seq文库制备试剂盒中的barcodes允许在Ion Torrent仪器上多达48个样本一起进行测序。如此高的混样程度可以节约成本如同QuantSeq特定长度节省测序空间一样。
QuantSeq-Pool结合了样本barcode标记和早期pooling与3' mRNASeq的优点,是一种节省时间、测序数据量和数据分析工作的基因表达谱分析方法。 这意味着与传统的mRNA-Seq项目相比,总成本降低了10倍。input量通常可以低至10 ng总RNA,内置的UMIs可以消除PCR带来的偏好性,从而实现高度可信、稳定的基因表达分析。便捷的操作流程...
QuantSeq is an oligodT primed NGS library preparation protocol generating only one fragment per transcript. This enables accurate gene expression quantification independent of the RNA quality (including FFPE samples). Standard mRNA-Seq protocols aim to cover the whole transcript, and result in a ...
QuantSeq is the gold-standard solution for gene expression studies targeting the 3’ end of RNA transcripts.
Bioinformatics Services: Leave the analysis to the RNA experts We offer a wide range of bioinformatics services ranging from standard analysis to fully custom data analysis projects. Our bioinformatics team consists of data analysis experts with experience in various NGS data analysis pipelines who are...
图1 | QuantSeq 3’ mRNA-Seq建库试剂盒(左侧)和Quant-Seq-Flex建库试剂盒(右侧) 工作流程图。QuantSeq FWD试剂盒(Cat No. 015)的Read 1反映了靠近3‘端的mRNA序列,实现了Illumina标准测序引物的经济的二代测序。在QuantSeq REV试剂盒中(Cat. No. 016),Read 1和Read 2的接头(adapter)位置互换,从而实现...
Figure 1: The QuantSeq (T-fill) workflow. From: QuantSeq 3′ mRNA sequencing for RNA quantification (a) Library generation and (b) amplification. (c) Sequencing using a customized sequencing primer (CSP) or T-fill reaction, both represented by T*. (d) Data processing. Back to article ...
QuantSeq 3′ mRNA sequencing for RNA quantification. Nature Methods. 2014;11(12).Moll P, Ante M, Seitz A, Reda T. QuantSeq 3 [prime] mRNA sequencing for RNA quantification. Nat Methods 2014;11.12....
M. (2018) "A Globin Blocker to Increase Sequencing Efficiency for QuantSeq 3' mRNA-Seq in Porcine Blood," Animal Industry Report: AS 664, ASL R3275. DOI: https://doi.org/10.31274/ans_air-180814-318 Available at: https://lib.dr.iastate.edu/ans_air/vol664/iss1/73...
Discover QuantSeq, the leading method for RNA analysis. QuantSeq offers fast, sensitive, and accurate gene expression profiling, even with low-quality samples.