The invention relates to a double fluorescent quantitative PCR kit used for detecting gene expression. The kit comprises PCR reaction liquid, DNA polymerase, a proof sample, a primer, a fluorescent probe, a primer and a fluorescent probe, wherein the primers and the fluorescent probes are used...
定量PCR 1. Quantitative PCR Techniques and Its Application Status; 定量PCR技术及其应用现状 2. Aquantitative PCRkit used in automatic genetic amplification instrument; 全自动(定量PCR)基因扩增诊断仪配套定量试剂的研制 3. Real-time fluorescencequantitative PCRand its application in animal nutrition; ...
在PCR循环的变性阶段,抗体受热被去除,聚合酶活性恢复,因此获得“热启动”功能,可减少残余DNA的污染,提高PCR扩增效率、灵敏度和目的片段产量。 SYBR Green I可以与DNA双链的小沟结合,结合后在497nm激发光照射下产生520nm的发射光。未结合双链DNA的SYBR Green I基本不产生荧光。因此可以根据荧光值的大小判断溶液内DNA...
本发明涉及一种基于ARMS荧光定量PCR检测基因突变的试剂盒和方法. The present invention relates to a kit and method based on ARMS PCR to detect the gene mutation. 与现有基因多态性检测方法相比,本发明的基于增强型ARMS荧光定量PCR检测基因多态性的试剂盒及方法不仅特异性强,敏感性高,成本低,结果高度可靠,背景...
Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six southern corn rootworm, Diabrotica undecimpunctata howardi
For instance, parenteral administration of non-tumorigenic cell DNA should be limited to 10 ng/dose and a maximum length of 200 bp, whereas less than 100 µg/dose of residual DNA is recommended for orally administered vaccines. 【Content】 Super cost-effective Run PCR NTC=No Template Control...
.3.7实时荧光定量PCR反应在73 型荧光PCR仪进行扩增。步骤:融解 所需试剂及标本 振荡混匀 短暂离心后 于光学专用8连管中配置反应体系。 IGH基因PCR扩增体系 模板DNA 2ul;上游引物 .3ul;下游引物 .3ul; SYBRGreenIRealtimePCRMasterMix 6.75ul;双蒸水 5.65ul 总体积 5ul. ...
A rapid an d reproducible method was first established for assessment of Hog cholera lapinized virus(HCLV)loads in Classical Swine Fever vaccine using Flurogenic quantitative PCR (FQ·PCR)combines LiIghtCycler sequence detection system.T... Y Chen,C Zhang,J Zhou,... 被引量: 6发表: 2003年...
EpiQuik PCR快速定量试剂盒能快速,特异,灵敏,可重复性实时定量分析DNA样本,特异的热启动聚合酶可以降低反应时间,结合试剂盒内量化试剂能保证定量PCR的特异性和稳定性,含有绿色荧光探针用于DNA的检测和分析,无需序列特异探针.
淋球菌核酸扩增(PCR)荧光定量检测试剂盒Neisseria Gonorrhoeae (NGH) Polymerase Chain Reaction(PCR)Fluorescence Quantitative Diagnostic Kit,中山大学达安基因股份有限公司,信息来源药智数据国产器械数据库。