qScript XLT One-Step RT-qPCR ToughMix, Low ROX is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan ® 5’-hydrolysis probes on Applied Biosystems 7500, 7500 Fast, ...
optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. qScript XLT One-Step RT-qPCR ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries....
Flexible - Use fast or standard qPCR cycling conditions. Broad dynamic range- Reliable data from your precious samples every time. Multiplexing enabled – Supports detection of up to four targets. qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse...
* Omit addition of qScript One-Step RT in minus RT control reactions.Reaction ProtocolIncubate the complete reaction mix in a real-time thermal detection system as follows:cDNA Synthesis Taq Activation PCR cycling (30 - 45 cycles)Fast qPCR Cycling 50°C, 5 min 95°C, 2 min 95°C, 3s ...