One Step PrimeScript III RT-qPCR Mix (Code No.: RR600A,200次,25 μl反应体系) ■ 制品说明 本制品是采用探针法进行1 Step Real Time RT-PCR的专用premix试剂。本制品将反转录酶和PCR酶及反应用buffer预先配制成premix形式,由于RT-PCR反应在单个反应管中连续进行,因而,操作更简便,同时降低了污染的风险。...
反转录酶采用了耐热性高的PrimeScript III RTase,且即使在反应阻害物存在的情况下也能够有效合成cDNA。此外,使用该试剂,也可以实现多个反应同时进行的多重qPCR。 5× One Step PrimeScript III RT qPCR Kit包含了比2× One Step PrimeScript III RT qPCR Mix(RR600)中更浓缩的premix。例如,25 μl反应只需要5 ...
Fast One Step RT-qPCR mix (2X), with UNG(Code No. TCH008/TCH012)的Premix中含有UNG (Uracil N-Glycosylase),可降解先前PCR反应的扩增产物,因此,对于重复检测特定目的基因等情况,可以防止PCR产物污染。反转录反应使用新型的PrimeScript III RTase,该酶在维持PrimeScript RTase的特异性和延伸性的同时,进一步提高...
One Step PrimeScriptTM Probe RT-qPCR Master Mix 中文名: 中文别名: CBNumber: CB99403818 分子式: 分子量: 0 MOL File: Mol file One Step PrimeScriptTM Probe RT-qPCR Master Mix化学性质 安全信息 One Step PrimeScriptTM Probe RT-qPCR Master Mix性质、用途与生产工艺 ...
Principle of one-step RT-qPCR Takara Bio'sOne Step PrimeScript III RT-qPCR Kitallows cDNA synthesis from RNA using PrimeScript Reverse Transcriptase, followed by PCR amplification with TakaraEx TaqHot Start Version in a single, uninterrupted procedure. PCR amplification products are detected and monito...
The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) is a quantitative real-time PCR kit designed for intercalating dye-enabled product detection. Using this TB Green Real-Time PCR (qPCR) kit, RT-PCR can be performed in a single tube, minimizing pipetting...
ABI 7500 thermocycler with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), GoTaq Probe 1- Step RT-qPCR System (Promega), LightCycler Multiplex RNA Virus Master (Roche) and One-step PrimeScript RT-PCR kit (Takara)....
ABI 7500 thermocycler with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), GoTaq Probe 1- Step RT-qPCR System (Promega), LightCycler Multiplex RNA Virus Master (Roche) and One-step PrimeScript RT-PCR kit (Takara)....
2. 使用TaKaRa Ex TaqHS、PrimeScript RT enzyme Mix Ⅱ时,应轻轻混匀,避免起泡;分取之前要小心地离心收集到反应管底部;由于酶保存液中含有50%的甘油,粘度高,分取时应慢慢吸取。 3. 2X One Step TB Green RT-PCR Buffer Ⅲ 融解后如有不溶物请充分混合。
TaKaRa一步法荧光染料法RT-qPCR试剂盒One Step TB Green®PrimeScript™RT-PCR Kit II是采用TB Green嵌合荧光法进行One Step Real Time RT-PCR反应的专用试剂。使用该试剂盒进行Real Time RT-PCR反应可在同一反应管内连续进行,操作简单,并能有效防止污染。该反应体系由于可以对扩增产物进行实时检测,大大提高了检...