商品名称:One step TB green prime script plus RT-PCR 规格:RR096B 品牌:TAKARA 特殊需求:- 货号:RR096B 单位:1 数量:1 供应商报价详情 序号:01 是否成交:成交 供应商名称:北京六合通经贸有限公司 报价时间:2023-03-13 09:39:27.0 最新...
Takara One Step PrimeScript™RT-PCR Kit 逆转录聚合酶链反应试剂盒 在线交易 24小时发货 少货必赔 破损包赔 北京百奥创新科技有限公司 3年 查看详情 ￥1570.40/盒 北京 Takara PrimeScript™ RT reagent Kit with gDNA Eraser 反转录试剂盒 在线交易 24小时发货 少货必赔 破损包赔 北京百奥创新科技有限公司...
品牌 翌圣生物 产品名称 4× Frag/Prime Buffer 产品规格 8 T 用途范围 仅做科研,不用于临床 加工定制 否 商品货号 13487ES 商品规格 25 mg 生产厂家 翌圣生物科技(上海)股份有限公司 是否进口 否 规格编码 13487ES08 最小起订量 1.00 发货地 上海 售卖区域 全国 可售卖地 全国 类型 科研试剂...
Genomic sequences flanking these mutations were obtained from RefSeq30 accessed October 2020, using the SPDI data model31 and a custom python script. The −10 to +4 bp region around the target sites were searched for NGG, NAN, or NGN PAMs. The efficiency of prime editing using NGG ...
The resulting merged fastq files were processed using a custom R script (read_match_pegRNAs.R, GitHub45). First, DNA sequences were trimmed to contain the 10 nt up- and downstream of the nick site (for target site amplicon) or to contain 15 nt up- and downstream of the nick ...
Quantitative RT-PCR showed loss of Tspan2 mRNA in aorta and bladder, but not heart or brain, of mice homozygous for an HDR-mediated three base pair substitution in the Tspan2 CArG box. Using the same protospacer, mice homozygous for a PE2-mediated single-base substitution in the Tspan2 CA...
产品名称 2 × Frag/Prime Buffer 产品规格 8T 用途范围 仅做科研,不用于临床 加工定制 否 商品货号 11377ES 商品规格 8T 生产厂家 翌圣生物科技(上海)股份有限公司 是否进口 否 规格编码 11377ES08 最小起订量 1.00 发货地 上海 售卖区域 全国 可售卖地 全国 类型 科研试剂 型号 11377ES ...
The pegRNAs or xr-pegRNAs were in vitro transcribed from T7 promoter-led templates using the MEGAshortscript™ T7 kit (Invitrogen). Their degradation profile under an endonuclease-inhibited condition was analyzed as recently described19, with minor modifications. Briefly, the nuclear extracts were...
Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses encoding for Cas9 and its respective guide RNA. However, transient RNP activity does not typically lead to optimal editing outcomes. Here we show that the efficiency of delivering RNPs can be enhanced by ce...
Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex ...