aRT-qPCR and western blotting were used to detect mRNA and protein expression of collagen type I (Col I), collagen type III (Col III), collagen type IV (Col IV), fibronectin and aldose reductase (AR). RTqPCR和西部弄脏用于查出胶原类型I Col I,胶原类型 (III Col) III,胶原类型 (IV Col)...
2. Burnette WN. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate...
RT-qPCR and western blotting were used to detect mRNA and protein expression of collagen type I (Col I), 方法: 鼠mesangial细胞 (RMCs) 被开化了得体外并且被划分了成六 (群控, Ang II, losartan和低,中间和高浓度lignans小组)。 RMC扩散由MTT分析用试样测量。 RTqPCR和西部弄脏用于查出胶原类型I Col...
然后作者沉默了缺氧诱导的ETS1过表达,并设计了两条siRNA fmiR-4521 expression was examined by qRT-PCR analysis in SGC7901-L and BGC-L cells transfected with ETS1 siRNAs during hypoxia (upper). Western blotting was performed to assess the inhibition efficiency in the same cells (lower). 然后作者...
microRNA qPCR 实验服务 ELISA 技术实验服务 Western Blotting 技术服务 蛋白芯片 技术服务(一) BioTNT 甲基化检测技术服务 基因分型技术(SNP)服务和产品解决方案 生化/其他试剂盒检测服务 更多>> 更多>> 技术宝典— qPCR qPCR 汇总(2)---Real time PCR mRNA 定量实验路线选择F&Q Real time ...
Validation of the microarray data for sample genes by qPCR and western blotting.Dadi JiangCatherine I. DumurH. Davis MasseyViswanathan RamakrishnanMark A. SublerJolene J. Windle
We offer a broad range of biological detergents and surfactants, including REACH-compliant biodegradable alternatives, for use in cell lysis, DNA/RNA electrophoresis, Western blotting, transfection, and many other life science research applications....
Western blot 实验技术 牛君星 定义: 印迹法(blotting)是指将样品转移到固相载体上,而后利用相应的探测 反应来检测样品的一种方法。 Western Blot是分子生物学、生物化学和免疫遗传学中常用的一种实验方 法。 1975年,Southern建立了将DNA转移到硝酸纤维素膜(NC膜)上,并利 用DNA-RNA杂交检测特定的DNA片段的...
NameBlotting,Western Tree numbersE05.196.401.143E05.301.300.096E05.478.566.320.200E05.601.262E05.601.470.320.200 E0402-02 | SG qPCR Master Mix(2x),外加用于定量实时PCR和两步实时RT-PCR的ROX溶液。含有氧化染料。尺寸:2X200反应| 328.00欧元 E0402-01 | SG qPCR主混合物(2x),加上用于定量实时PCR和两...
这时候要冷静的观察自己的实验设置，比如基因很短时，很多软件会犯傻，基因有同源基因家族时，很多软件...