The detection of aRNAs and qiRNAs in WT and other mutant lines after UV treatment by Northern blot and RT-qPCR analysis.Hui ChenKappei KobayashiAkio MiyaoHirohiko HirochikaNaoto YamaokaMasamichi Nishiguchi
The southern blot probe was generated using the Prime-It II Random Primer Labeling Kit (Agilent, #300385) with alpha-dCTP32 on a 689 bp PCR product (part of dTomato sequence) amplified with AmpliTaq-Gold 360 DNA Polymerase (ThermoFisher, #4398790) from the coMIP247 plasmid using primers ...