QIAquick PCR Purification Kit and QIAquick PCR & Gel Cleanup Kit Quick-Start Protocol - (EN) EN PDF(571KB) Safety Data Sheets (1) Safety Data Sheets EN Certificates of Analysis (1) Certificates of Analysis EN FAQ I bound an 11 kb DNA fragment to a QIAquick column; is it com...
The QIAprep Spin Miniprep Kit or QIAwave Plasmid Miniprep Kit enables purification of up to 20 µg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep 2.0 Spin Columns can be used ...
For multiplex PCR, 1 µl of eluate (approximately 30 ng DNA) was used as template. Multiplex PCR of the amelogenin gene and the STR markers vWA, D7S820, F13A1, HUMTH01, and FES/FPS was performed using the QIAGEN Multiplex PCR Kit and the protocol for amplification of microsatel- ...
protocol can be downloaded at The RNeasy Kits are designed to purify RNA from small amounts of starting material They provide a fast and simple method for preparing up to 100 g total RNA per sample The purified RNA is ready for use in downstream applications such as RT PCR and real time...
原理QIAamp DNA Microbiome Kit是进行微生物组分析的专用样品制备解决方案,这是因为它能在有效清除宿主DNA的同时,最大限度控制混合样品中细菌群落的组分检测偏差。其结果就是能够获得具有更高覆盖度的细菌DNA富集产物。这一优点得益于该试剂盒的诸多特征。首先,QIAamp DNA Microbiome Kit使用了Ultra Clean Production(UCP...
They provide a fast and simple method for preparing up to 100 g total RNA per sample. The purified RNA is ready for use in downstream applications such as: RT-PCR and real-time RT-PCR Differential display cDNA synthesis Northern, dot, and slot blot analyses Primer extension Poly A+RN 40...
Residual ethanol in the eluate may cause inhibition of PCR leading to false-negative results. 11. Place the QIAamp Maxi column in a clean 50 ml centrifuge tube (provided), and discard the collection tube containing the filtrate. Note: Use a wet tissue paper to wipe any spillage off the ...
is highly suitable for use in miRNA quantification by real-time RT-PCR. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. This specialized protocol requires use of the RNeasy MinElute Cleanup Kit ...
‘wash and spin’ steps to precipitate clean DNA and get rid of cell debris in the form of polysaccharides, proteins and salts. The DNeasy kit makes it easier to generate clean DNA which is important for PCR applications, since salt contamination can hinder the efficiency of DNA amplification...
purified PCR products ranging from 350 bp to 1 kb, all from a 2.0% agarose TBE gel. The protocol for agarose gel extraction involves first running the DNA to be purified on a suitable gel and then excising the DNA bands into a microcentrifuge tube with a clean scalpel. Each band must ...