The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional...
DNA 提纯/清理试剂(DNA clean-up kit) QIAquick PCR Purification Kit和QIAquick Gel Extraction Kit 显然,PCR purification kit是用来清理PCR反应液中可能剩下的蛋白质、阳离子和dNTPs,而Gel extraction kit则可以从琼脂胶中提纯DNA(电泳过后,提取指定条带)。两种试剂盒中提供的洗涤液和洗脱液基本相同,而且他们使用...
QIAprep Spin columns bind up to 20 ug of plasmid DNA up to 50 kb in length, while the QIAquick Spin columns bind up to 10 ug of DNA with a maximum fragment size of 10 kb. FAQ ID -311 Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Remova...
QIAGEN OneStep RT-PCR Kit提供方便的规格,可使用RNA模板进行高灵敏度和特异性的RT-PCR。试剂盒中包含优化的组分,可在同一反应混合液中进行逆转录和PCR扩增,完成”一步式”反应。du特的酶组合和专门研发的反应缓冲液确保在一个管中进行高效、高特异性的逆转录和PCR,无需优化(参见Highly specific RT-PCR using lo...
180795 QIAseq CleanStart PCR Kit (100) 100Reactions 3640 Qiagen 181703 QIAseq Single Cell DNA Lib Kit UDI (24) 24Reactions 13500 Qiagen 181705 QIAseq Single Cell DNA Lib Kit UDIA (96) 96Reactions 50000 Qiagen 181707 QIAseq Single Cell DNA Lib Kit UDI (384) 384Reactions 180000 Qiagen 181725...
QIAquick PCR Purification Kit (250) 5150 28106 QIAquick 96 PCR Purification Kit (4) 10180 28181 QIAquick 96 PCR Purification Kit (24) 40340 28183 MinElute Reaction Cleanup Kit (50) 1600 28204 MinElute Reaction Cleanup Kit (250) 7340 28206 QIAquick Nucleotide Removal Kit (50) 1410 28304 QI...
The high-quality RNA allows sensitive and precise downstream analysis using methods such as microarray analysis (Figure 2) and real-time RT-PCR (Table 1). Up to 1 x 106 cultured cells, 2 x 106 blood cells, or 10 mg easy-to-lyse tissue can be processed per sample. Yields range from ...
DNA clean up Elute ds-cDNA in 72ul elution buffer BioPrime labeling For 25-500ng template DNA ΦX174 template DNA (labeled): 25ng (1.9ug), 100ng (2.5ug), 500ng (3.4ug) Contaminants in template DNA will result in difficulties of labeling In 200ul PCR tube, add on ice: 72ul DNA...
The QIAprep Spin Miniprep Kit or QIAwave Plasmid Miniprep Kit enables purification of up to 20 µg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep 2.0 Spin Columns can be used ...
up to 100 g total RNA per sample The purified RNA is ready for use in downstream applications such as RT PCR and real time RT PCR Differential display cDNA synthesis Northern dot and slot blot analyses Primer extension Poly A RNA selection RNase S1 nuclease protection Microarrays The RNeasy ...