Puromycin labeling does not allow protein synthesis to be measured in energy-starved cellsMammalian evolution exhibits extraordinary acceleration in phenotypic complexity while displaying genetic conservation. Indeed, protein-coding genes are 90% identical between mice and human, and the question arises what...
产品活性:Puromycin dihydrochloride (CL13900 dihydrochloride) 是一种氨基核苷类抗生素,抑制蛋白合成 (protein synthesis)。 研究领域:Anti-infection 作用靶点:Bacterial | Antibiotic In Vitro: Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of...
Puromycin modified with another molecule is incorporated into a protein at the C-terminus, thus linking the desired molecule to the protein. Combination of in vitro translation with puromycin analogues has resulted in novel technologies such as display technology for screening, fluorescence labeling, ...
PuNCH‐P delivers an estimate of the rate of protein production using a technique complementary to ribosome footprinting or polysome loading. This method circumvents the need for in‐vivo labeling such as in SILAC or BONCAT methods, ... A Noras,AVV Arnim - 《Faseb Journal》 被引量: 0发表:...
3.4Protein labeling and formation of mRNA-peptide fusions At low non-inhibitory concentrations, about two orders of magnitude less than those commonly used for selection,puromycincannot effectively compete with aa-tRNA and therefore only becomes incorporated when ribosomes reach astop codon, generating ...
产品活性:Puromycin dihydrochloride (CL13900 dihydrochloride) 是一种氨基核苷类抗生素,抑制蛋白合成 (protein synthesis)。 研究领域:Anti-infection 作用靶点:Bacterial | Antibiotic In Vitro: Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of...
Classical pulse-chase or flooding dose methods used to monitor protein synthesis rely on the measurement of radioactive methionine and cysteine labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling, and allows for the evaluation/quantification of...
How does puromycin inhibit protein synthesis?Puromycin, an antibiotic, is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biolo.....
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In an effort to improve the visualization of protein synthesis under in vivo conditions, a 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-labeled derivative of Pur was prepared for labeling with radionuclides such as (68)Ga or (44)Sc, which can be used with positron ...