Also for a selection of proteins increasing over time upon drug treatment, we found up-regulated transcripts and a higher level of de novosynthesis; (5) Our novel pcSILAC strategy may assist in studying drug modes of action, drugs effect on protein turnover, and more broadly, it could be ...
The SILAC analyses of HeLa cells indicated that assembly of RCV, comprising F1/Fo-ATPase, is rapid with little excess subunit synthesis, but that assembly of RCI (i.e. NADH dehydrogenase) is far less efficient, with dramatic oversynthesis of numerous proteins, particularly in the matrix-exposed...
Fierro-Monti I, Racle J, Hernandez C et al (2013) A novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor. PLoS One 8(11):e80423. : 10.1371/journal.pone.0080423 Central...
Determination of Cytokine Signaling-Dependent Protein Stability by Pulse-Chase SILAC Experiments1. Cytokine withdrawal is associated with changes in protein halflives. 2. Changes in protein stability occurring upon cytokine withdrawal are providing novel insights into the regulatory processes underlying early...