Pulse-chase SILAC detects global, simultaneous differential changes in protein degradation and synthesis affecting proteostasis and cell signaling upon Hsp90 inhibition.(1) The plethora of multiple dynamic effects observed in the clusters analysed could be considered a paradigm of mechanisms operating to ...
We report here that pulse-chase stable isotope labeling with amino acids in cell culture (SILAC) is a valuable technique to study RC assembly because it can reveal considerable differences in the assembly rates and efficiencies of the different complexes. The SILAC analyses of HeLa cells indicated...
Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (‘pulse’) of a kinase of interest, as well as global and in-depth characterization (‘chase’) of the perturbed proteome under living conditions. Here we developed an optogenetic ‘pulse-chase’ ...
Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (‘pulse’) of a kinase of interest, as well as global and in-depth characterization (‘chase’) of the perturbed proteome under living conditions. Here we developed an optogenetic ‘pulse-chase’ ...
Fierro-Monti I , Racle J , Hernandez C , Waridel P , Hatzimanikatis V , Quadroni M ( 2013 ) A novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor . PLoS ONE 8 : e80423...
Determination of Cytokine Signaling-Dependent Protein Stability by Pulse-Chase SILAC Experiments1. Cytokine withdrawal is associated with changes in protein halflives. 2. Changes in protein stability occurring upon cytokine withdrawal are providing novel insights into the regulatory processes underlying early...