Combining pulsed-SILAC labeling and click-chemistry to probe rapid proteome dynamics - an application to macrophage activationA novel approach is presented for the detection of proteome cha scale (<1h). This should provide a useful tool to study immedia perturbation as shown for LPS-activation here...
In this investigation we used pulsed SILAC to analyze which cellular pathways are immediately affected by treatment of HeLa cells with Sunitinib.Robert LJ GrahamMichael J SweredoskiSonja HessProceedings 59th ASMS Conference on Mass Spectrometry and Allied Topics: June 5 - 9, 2011. Denver, CO...
BONCATPulsed SILACQuantitative analysisSecreted proteinsSecretomeSerum-containing mediumSecreted proteins play pivotal roles in signal transduction and cell-to-cell communication. Despite increasing interest in secretome analysis over the past decade, most studies on this topic have utilized serum-free medium ...
Pulsed SILACQuantitative mass spectrometrymicroRNA targetsDe novo protein synthesismiR-34ap53Pulsed stable isotope labeling by amino acids in cell culture (pulsed SILAC or pSILAC) allows to monitor and quantify the de novo synthesis of proteins in an unbiased fashion on a proteome-wide......
(2009) Global analysis of cellular protein translation by pulsed SILAC. Proteomics 9, 205-209.Schwanhausser B, Gossen M, Dittmar G, et al. (2009). Global analysis of cellular protein translation by pulsed SILAC. Proteomics 9, 205-209....
Dynamics of zebrafish fin regeneration using a pulsed silac approach. Nolte H HS,Housley MP,Islam S,et al. Proteomics . 2014Nolte H, Holper S, Housley MP, Islam S, Piller T, Konzer A, et al. (2015) Dynamics of zebrafish fin regeneration using a pulsed SILAC approach. Proteomics 15: ...
In vivo pulsed SILAC labeling reveals distinctive, age-dependent, turnover rates of collagens of articular cartilage and boneSupporting Table of SILAC analysis. (XLSX)doi:10.1016/j.joca.2018.02.077Ariosa-Morejon Y.Charles P.Davis S.Fischer R....