In this kind of assay, a recombinant affinity tag fusion protein is used as bait to capture (‘pull down’) binding partners out of a cell lysate. The cell lysate is applied to the immobilised bait protein, or, alternatively, bait protein and lysate are mixed in solution and complexes are...
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In this review, we will focus on the detailed methodology of SiMPull, its salient features and a wide range of biological applications in comparison with other biosensing tools. 展开 关键词: co‐immunoprecipitation fluorescence protein complex pull‐down single molecule Western blotting ...
Ampicillin/penicillin‐binding protein interactions as a model drug‐target system to optimize affinity pull‐down and mass spectrometric strategies for ta... E.,Xu, N. F.,Kery, V.Ampicillin/penicillin-binding protein interactions as a model drug-target system to optimize affinity pull-down and ...
The aim of this unit is to provide a method for the identification of new protein‐protein interactions. Pull‐down experiments with GST fusion proteins at... A Brymora,VA Valova,PJ Robinson - John Wiley & Sons, Inc. 被引量: 85发表: 2004年 AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD...
英文别名: SNAP‐CAPTURE PULL‐DOWN RESIN中文名: 中文别名: CBNumber: CB44691758 分子式: 分子量: 0 MOL File: Mol file 化学性质 安全信息 用途 供应商 8 SNAP‐CAPTURE PULL‐DOWN RESIN化学性质 安全信息SNAP‐CAPTURE PULL‐DOWN RESIN性质、用途与生产工艺 SNAP‐CAPTURE PULL‐DOWN RESIN 上下游产品信息...
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The optimized probes could selectively pull down CA from red blood cell lysate and other protein mixtures. Pull-down of differently metallated CAs was also investigated. 展开 关键词: carbonic anhydrase desthiobiotin metalloproteins proteomics sulfonamide ...