Phosphorylation of O6-alkylguanine-DNA alkyltransferase: experience with a GST-fusion protein and a new pull-down assay We showed recently that human O 6-alkylguanine-DNA alkyltransferase (AGT), a key target for enhancing the efficacy of anticancer alkylating agents, is regu... KS Srivenugopal...
In this kind of assay, a recombinant affinity tag fusion protein is used as bait to capture (‘pull down’) binding partners out of a cell lysate. The cell lysate is applied to the immobilised bait protein, or, alternatively, bait protein and lysate are mixed in solution and complexes are...