PTBP1 蛋白的 4 个不同 RRM,通过结合同一 mRNA 的不同位点,来重构 mRNA 的结合底物,并且将mRNA 形成 RNA loop 结构。拟南芥 RRM1 结构域由 PTBP1 肽链第 17 到 95 位氨基酸残基形成;RRM2 结构域由 PTBP1 肽链第109 到 196 位氨基酸残基形成;RRM3 结构域由 PTBP1 肽链第242 到 322 位氨基酸残基形成。
The small RNAs were found to be derived from the 3′ stem regions of Y RNA via cleavage of the single-strand loop region (Fig. 1A). Fig. 1: Y RNA cleavage is activated in apoptotic cells. A Common motif of ASRs was identified by MEME motif search. The motif, indicated in the ...
To test if PTBP1 recruitment near the 5′ end of intron 6 was sufficient for splicing activation, we inactivated all five Py sequences and simultaneously integrated three bacteriophage MS2-specific stem-loop sequences at the Py1–3 positions (Figure 5A). We then co-transfected A2lox ESCs with...
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Tumor size was measured using an electronic calliper and calculated using the formula 4π/3 x r3 (radius (r) calculated from the average diameter, measured as the tumor width and length). Optical imaging was performed using the IVIS SpectrumCT system. All animal work followed institutional ...
CircPDIA3/miR-449a/XBP1 feedback loop curbs pyroptosis by inhibiting palmitoylation of the GSDME-C domain to induce chemoresistance of colorectal cancer. Drug Resist Updat. 2024;76:101097. Article CAS PubMed Google Scholar Mihailovich M, Bremang M, Spadotto V, Musiani D, Vitale E, Varano ...
pre-mRNA. Conversely, ITSN1 transcript was detected in the cDNA library of the PTBP1 RIP, and these results revealed that PTBP1 bound to the sequence between 4F and 4R in exon 30 ofITSN1pre-mRNA with higher affinity (Fig.4E). Meanwhile, RT–qPCR results showed that ITSN1 RNA was ...
PTBP2 may also be involved in this autoregulatory loop via a positive feedback mechanism. Figure 6 Model of SRSF3 expression regulated by PTBP1 and PTBP2 via controlling the alternative splicing of exon 4. (A) The exon 4-excluded transcripts encode full length functional SRSF3. The exon ...