PTBP1 蛋白的 4 个不同 RRM,通过结合同一 mRNA 的不同位点,来重构 mRNA 的结合底物,并且将mRNA 形成 RNA loop 结构。拟南芥 RRM1 结构域由 PTBP1 肽链第 17 到 95 位氨基酸残基形成;RRM2 结构域由 PTBP1 肽链第109 到 196 位氨基酸残基形成;RRM3 结构域由 PTBP1 肽链第242 到 322 位氨基酸残基形成。
[2] Spellman R, Llorian M, Smith CW. Crossregulation and functional redundancy between the splicing regulator PTB and its paralogs nPTB and ROD1[J]. Mol Cell, 2007, 27(3): 420-434. DOI:10.1016/j.molcel.2007.06.016 [3] Kim W, Shin J...
The small RNAs were found to be derived from the 3′ stem regions of Y RNA via cleavage of the single-strand loop region (Fig. 1A). Fig. 1: Y RNA cleavage is activated in apoptotic cells. A Common motif of ASRs was identified by MEME motif search. The motif, indicated in the ...
To test if PTBP1 recruitment near the 5′ end of intron 6 was sufficient for splicing activation, we inactivated all five Py sequences and simultaneously integrated three bacteriophage MS2-specific stem-loop sequences at the Py1–3 positions (Figure 5A). We then co-transfected A2lox ESCs with...
which makes us more supportive of the latter hypothesis. It is possible that the reason for the discrepancy in these findings is the existence of a parallel relationship between PTBP1 and c-Myc, and the intermediate feedback loop needs to be filled by further findings. Altogether, our findings...
2 For simplicity, we represent each copy of PTBP1 bound to each side of the RNA loop, and the loop is stabilized through PTBP1 dimerization. It is important to note, though, that PTBP1 contains multiple RNA-binding domains, and thus different domains of a single PTBP1 molecule could ...
et al. The FUS/circEZH2/KLF5/ feedback loop contributes to CXCR4-induced liver metastasis of breast cancer by enhancing epithelial-mesenchymal transition. Mol Cancer. 2022;21:198 55. Fu Z, Zhang P, Zhang R, Zhang B, Xiang S, Zhang Y. et al. Novel hypoxia-induced HIF1alpha-circTDR...
CircPDIA3/miR-449a/XBP1 feedback loop curbs pyroptosis by inhibiting palmitoylation of the GSDME-C domain to induce chemoresistance of colorectal cancer. Drug Resist Updat. 2024;76:101097. Article CAS PubMed Google Scholar Mihailovich M, Bremang M, Spadotto V, Musiani D, Vitale E, Varano ...
Y Kato,R Kusakabe,K Inoue,S Tochinai 摘要: MicroRNAs (miRNAs) comprise a group of small noncoding RNA molecules thought to have contributed to the evolution of vertebrate brain homogeneity and diversity. The miRNA miR-124 is well conserved between invertebrates and vertebrates and is expressed ...
CircPDIA3/miR-449a/XBP1 feedback loop curbs pyroptosis by inhibiting palmitoylation of the GSDME-C domain to induce chemoresistance of colorectal cancer. Drug Resist Updat. 2024;76:101097. Article CAS PubMed Google Scholar Mihailovich M, Bremang M, Spadotto V, Musiani D, Vitale E, Varano ...