The rapid proximity labeling system PhastID identifies ATP6AP1 as an unconventional GEF for RhebRheb is a small G protein that functions as the direct activator of the mechanistic target of rapamycin complex 1 (mTORC1) to coordinate signaling cascades in response to nutrients and growth factors. ...
Introduction Plants have a sophisticated immune system to defend against pathogen attack. RNA silencing is viewed as a primary antiviral defense system of plants1. Most plant viruses have RNA genomes and form double-stranded viral RNA (dsRNA) structures or dsRNA replicative intermediates, which can t...
For LC-MS/MS analysis, tryptic peptides were separated by a 135 min gradient elution at a flow rate 0.300 µL/min with the DIONEX ultimate 3000 integrated nano-HPLC system (Thermo Fisher) which is directly interfaced with the Thermo LTQ-Orbitrap mass spectrometer. The analytical column...
Conlan, B., Stoll, T., Gorman, J. J., Saur, I. & Rathjen, J. P. Development of a rapid in planta bioid system as a probe for plasma membrane-associated immunity proteins.Front. Plant Sci.9, 1882 (2018). Roux, K. J., Kim, D. I., Burke, B. & May, D. G. BioID: a...
Quantitative LC/MS/MS was performed on 2 μl of each sample, using a nanoAcquity UPLC system (Waters Corp) coupled to a Thermo QExactive Plus high resolution accurate mass tandem mass spectrometer (Thermo) via a nanoelectrospray ionization source. Briefly, the sample was first trapped on a ...
Using the recombinant VSV system in combination with glycoproteins from different viruses, including MERS and CHIKV, we could show a broader dependency of viral entry on LARP1 expression. Interestingly, full-length infection with CHIKV increased viral replication in the absence of LARP1. Similar ...
elegans . We use this protocol to characterise the proteomes of the worm's gut, muscle, skin, and nervous system. We express TurboID exclusively in the pair of AFD neurons and show we can identify known and previously unknown proteins expressed selectively in AFD. We knock TurboID into the ...
P. Development of a rapid in planta BioID system as a probe for plasma membrane-associated immunity proteins. Frontiers in Plant Science. 9 (1882), (2018). Macharia, M. W., Tan, W. Y. Z., Das, P. P., Naqvi, N. I., Wong, S. M. Proximity-dependent biotinylation screening ...
The properties of the fastest growing oblique modes are examined in terms of the system parameters and compared to those of the dominant two-stream and filamentation modes 展开 关键词: a unified manner previously published beam-plasma system ...
now report a system that allows rapid nontoxic proximity labeling in just 10 minutes. Using yeast display, they evolved the Escherichia coli biotin ligase BirA to generate new promiscuous tagging variants, which they dubbed TurboID and miniTurbo. The researchers demonstrate that the TurboID system ...