Simon Fredriksson以第一作者在Nat Biotechnol期刊上发表题为“Protein detection using proximity-dependent DNA ligation assays”的文章[1],改文章主要介绍了提出了通过两个DNA适配子与目标蛋白的结合,促进寡核苷酸连接到每个适配子亲和探针,两个这样的近端探针连接产生一个可扩增的DNA序列,可扩增DNA序列的数量反映目标...
Proximity ligation assays: a recent addition to the proteomics toolbox An essential skill for every researcher is to learn how to select and apply the most appropriate methods for the questions they are trying to answer. With ... I Weibrecht,KJ Leuchowius,CM Clausson,... - 《Expert Rev ...
Fredriksson, S., Gullberg, M., Jarvius, J.et al.Protein detection using proximity-dependent DNA ligation assays.Nat Biotechnol20, 473–477 (2002). https://doi.org/10.1038/nbt0502-473 Download citation Received02 July 2001 Accepted22 February 2002 ...
www.nature.com/scientificreports OPEN Visualization of human karyopherin beta-1/importin beta-1 interactions with protein partners in mitotic Received: 28 April 2017 Accepted: 29 December 2017 Published: xx xx xxxx cells by co-immunoprecipitation and proximity ligation assays Laura Di Francesco1,4, ...
(33). In the present work, we have explored potential homo- and heteromeric interactions among mammalian HAS iso- forms incells insituusing fluorescence resonance energy trans- fer (FRET) and proximity ligation assays (PLA). The results相关...
In Drosophila, the male-specific lethal (MSL) complex specifically targets the male X chromosome and participates in a twofold increase in expression outpu
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Size-based separations as an important discriminator in development of proximity ligation assays for protein or organism detection Electrophoresis, 31 (2010), pp. 1615-1622 CrossrefView in ScopusGoogle Scholar [6] D. Shah, M.D. Dang, R. Hasbun, et al. Clostridium difficile infection: update ...
The proximity ligation assay (PLA) has previously been used for the sensitive and specific detection of single proteins. In order to adapt PLA methods for the detection of cell surfaces, we have generated multivalent peptide–oligonucleotide–phycoerythrin conjugates ('burrs') that can bind adjacent ...