Simon Fredriksson以第一作者在Nat Biotechnol期刊上发表题为“Protein detection using proximity-dependent DNA ligation assays”的文章[1],改文章主要介绍了提出了通过两个DNA适配子与目标蛋白的结合,促进寡核苷酸连接到每个适配子亲和探针,两个这样的近端探针连接产生一个可扩增的DNA序列,可扩增DNA序列的数量反映目标...
Protein detection using proximity-dependent DNA ligation assays， Nature Biotechnology 20, 473 - 477 (2002) 2） Solid phase PLA （固相PLA） 在液相PLA的基础上引入了一个捕获抗体，该捕获抗体被固定在固相载体上（磁珠、微孔板等），当目标蛋白被捕获后，通过Proximity Probe进行识别和定量。Detection of ...
分类： science Proximity Ligation Assay is a technology that extends the capabilities of traditional immunofluorescence and immunohistochemistry to include direct detection of proteins-protein interactions and modifications with unparalleled specificity and sensitivity. Target proteins are detected with single ...
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Proximity ligation assayProximity extension assayIn situ assaysProximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a ...
Proximity ligation assayThe present invention relates to compositions and methods for sensitive, rapid and convenient assays to detect and/or quantify one or more target cell or tissue using ribonucleic acid as probes, wherein the method includes binding a first and a second ribonucleic acid probe,...
α-Synuclein proximity ligation assay (PLA) has proved a sensitive technique for detection of non-Lewy body α-synuclein aggregate pathology. Here, we describe the MJF-14 PLA, a new PLA towards aggregated α-synuclein with unprecedented specificity, usin
Proximity ligation assays: a recent addition to the proteomics toolbox proximity ligation assayAn essential skill for every researcher is to learn how to select and apply the most appropriate methods for the questions they are... I Weibrecht,KJ Leuchowius,CM Clausson,... - 《Expert Rev Proteo...
PROXIMITY LIGATION ASSAY
in situ proximity ligation assay (PLAprotein‐protein interaction (PPIsignaling pathwayUnderstanding signaling pathway networks via protein-protein interactions (PPIs) at the cellular level is a significant task that has not yet been completed. Here, a systems approach that computationally infers ...