邻近连接分析,英文Proximity ligation assay,简称PLA, 中文翻译为邻位连接分析或者邻近连接分析技术。 邻近连接分析PLA是一种特殊的免疫分析方法,可用于检测目标蛋白,蛋白相互作用等等。 邻近连接分析PLA由Fredriksson等人于2002年开发。到目前为止,它已成为一种成熟且普遍适用的免疫组化工具,用于高级和精确的蛋白质分析。
Henrik H. J. Persson为通讯作者在Proc Natl Acad Sci U S A期刊上发表题为“Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies”的文章[5],提出了一种高度特异的环状邻近连接试验(c-PLA),它在严格性、易用性和与低亲和力试剂的相容性方面优...
location in tissue samples offers exciting new opportunities in biomarker research. By providing the element of dual recognition to in situ analyses, pathology can expand into a new arena of analytes, beyond measuring the abundance of just a single protein.PLA™, a proximity ligation assay ...
邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 发布邻近连接技术-proximity ligation assay (PLA),检测低丰度蛋白互作的方法 剧情介绍:
PLA™,aproximityligationassaytechnology,iscapableofdetectingsingleproteineventssuch asproteininteractions(e.g.proteindimerization)andmodifications(e.g.proteinphosphorylation). TheDuolink®reagentseries,basedonPLA,offersanunprecedentedlevelofsensitivityand ...
The proximity ligation assay (PLA) employs two or more affinity reagents, such as aptamers (Fredriksson Nat Biotechnol 20:473, 2002) or more commonly antibodies (Gullberg PNAS 101:8420, 2004; Schallmeiner Nat Methods 4:135, 2007), to detect a target protein or protein complex. The affinity ...
Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay. The activity of proteins is typically regulated by secondary modifications and by interactions with other partners, resulting in the formation of protein c... O S?Derberg,KJ Leuchowius,M...
Proximity Ligation Assay (PLA)简介 Proximity Ligation Assay (PLA)简介 1, 什么是PLA技术?PLA技术全称为Proximity Ligation Assay， 国内翻译为邻位连接或者邻近连接技术。PLA技术是一种特殊的免疫分析方法，可用于检测目标蛋白，蛋白相互作用等等。 该方法通过一对标记有一段寡聚脱氧核苷酸(单链DNA)的单克隆或者多...
PROXIMITY LIGATION ASSAY 专利名称：PROXIMITY LIGATION ASSAY 发明人：Matthew Levy,Andrew D. Ellington,Supriya Pai 申请号：US11834546 申请日：20070806 公开号：US20080293051A1 公开日：20081127 专利内容由知识产权出版社提供 专利附图：摘要：The present invention relates to compositions and methods for sensitive...