Here we report the identification of critical components for a ubiquitin/proteasome-dependent INM protein degradation pathway in plants using proximity-labeling proteomics. This pathway raises an important parallel with ERAD, CHLORAD, and LDAD pathways, all of which exploit the CDC48 complex. It ...
In comparison to the conventional methods to probe the PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach to characterize PPIs. However, the application of PL in planta remains at infancy...
Proximity Labeling(PL),邻近标记 原理 将一个具有邻近标记功能的酶(PL ligase)与目标蛋白(诱饵蛋白,Bait protein)融合,通过酶催化的共价修饰将邻近的蛋白标记上生物素(biotin),最后通过亲和素磁珠富集生物素标记蛋白进行质谱鉴定,分析目的蛋白的互作或邻近的蛋白信息。 Mathew B, et al. Mol Cell Proteomics. (202...
Major efforts have been made during the past 25 years to characterize invadopodia molecular composition, using classical targeted proteomics involving cell fractionation or co-immunoprecipitation after cell lysis or candidate approaches. These studies have established that invadopodia are composed of structural...
Unraveling Plant Nuclear Envelope Composition Using Proximity Labeling Proteomics Yu Tang&Yangnan Gu,2025,Springer Protocols In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation Joselyn S. Sotoet al.,2023,Nature Protocols ...
Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane-Bound Protein Clusters.doi:10.1002/0471140864.ps1927s80SPPLATproteinmicroenvironmentsproteomicsproximityquantitative...
Proteomics of primary cilia by proximity labeling Dev. Cell, 35 (2015), pp. 497-512 Google Scholar Nguyen et al., 2017 T.B. Nguyen, S.M. Louie, J.R. Daniele, Q. Tran, A. Dillin, R. Zoncu, D.K. Nomura, J.A. Olzmann DGAT1-Dependent lipid droplet biogenesis protects mitochondr...
- 《Molecular & Cellular Proteomics》 被引量: 15发表: 2015年 Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2 BP labelling occurs in 1min, providing temporal control of labelling. The APEX2 tool enables proteomic mapping of subcellular compartments as well...
Proximity-dependent labeling has proven to be a valuable method for revealing protein-protein interaction networks in living cells. A mutant form of the biotin protein ligase enzyme from Aquifex aeolicus (BioID2) underpins this methodology by producing biotin that is attached to proteins that enter ...
184Citations 107Altmetric Metrics Abstract This protocol describes the use of TurboID and split-TurboID in proximity labeling applications for mapping protein–protein interactions and subcellular proteomes in live mammalian cells. TurboID is an engineered biotin ligase that uses ATP to convert biotin int...