Using label free proteomics, we developed a strategy for the identification and quantification of plasma proteins in control samples. Heparinised plasma from three healthy volunteers (two women, one man) was obtained. 40 渭L plasma was immunodepleted with high performance liquid chromatography (HPLC...
Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import.Nat Commun.2018 Sep. 本文通过SiRNA技术在人细胞中构建Sec61和TRAP敲除细胞模型,通过Label free 蛋白组学技术分析差异蛋白,寻找TRAP依赖性多肽,并在细胞模型和CDG病人细胞中进行WB和qPCR验证。结果...
Label-free quantitativeproteomics(Labelfree)technology 概述: 定量蛋白质组学是蛋白质组研究的重要内容,通过对基因表达产物——蛋白质的定量分析,以了解基因在不同的生理、病理和逆境条件下的表达情况。近年来,随着非凝胶技术的发展,“鸟枪法”蛋白质组学(“Shotgun”proteomics)技术,特别是多维蛋白质鉴定(Multidimension...
Biopharmaceutical Perspectives Biopharmaceuticals and Protein Analysis Cannabis Analysis Capillary Electrophoresis Chiral Chromatography ChromAcademy Corporate Profiles Data Acquisition, Handling, and Archiving Data Analysis, Statistics, and Chemometrics Dietary Supplements Analysis ...
We have launched 4D label-free proteomics services to help research in microsample proteomics and larger cohorts of clinical samples. These services are accompanied by a high-quality full after-sales service.
参考文献: 1. Fabre B, Korona D, Groen A, et al. Analysis of Drosophila melanogaster proteome dynamics during embryonic development by a combination of label‐free proteomics approaches[J]. Proteomics, 2016, 16(15-16): 2068-2080....
图2 | label free流程图 参考文献Zhu, W., J. Smith and C. Huang. “Mass Spectrometry-Based Label-Free Quantitative Proteomics.” Journal of Biomedicine and Biotechnology 2010 (2010): n. pag.TMT/iTRAQ标记定量蛋白组学 就拿其中的一个来说：TMT全称是Tandem Mass Tag，是赛默飞公司的经典化学标记...
*Label-free定量蛋白质组学技术原理* *Label-free技术流程及应用场景* *Label-free蛋白质互作筛选应用案例* 参考文献: Molecular & cellular proteomics 2014, 13(9):2513-2526. (MaxLFQ原创文献,几乎是label-free实验的标准方法和分析流程)
3) A label-free differential proteomics analysis reveals the effect of melatonin on promoting fruit ripening and anthocyanin accumulation upon postharvest in tomato. 4) Integrative Transcriptome and Proteome Analysis Identifies Major Metabolic Pathways Involved in Pepper Fruit Development400...
发表期刊:Journal of Proteomics 发表时间:2021 影响因子:3.509 作者单位:浙江农林大学 涉及的百趣服务产品:Label free定量蛋白组学、GC-TOF-MS代谢组学 样本类型:叶片 主要研究内容: 油菜菌核病是一种常见的油菜病害,由植物坏死病原菌核盘菌引起。褪黑激素(MT)具有已知的生物活性,能有效缓解油菜的这种菌核病。文章...