Using label free proteomics, we developed a strategy for the identification and quantification of plasma proteins in control samples. Heparinised plasma from three healthy volunteers (two women, one man) was obtained. 40 渭L plasma was immunodepleted with high performance liquid chromatography (HPLC...
由于Label free技术无需进行繁琐的标记定量前处理,对于样品的损耗较少,也不会受到标记试剂标签数量的限制。因此,对于微量样品(如外泌体、co-IP产物、修饰蛋白组)、大批量队列样品(如血浆、唾液)以及Fast Proteomics(Biomarker研究)的检测,Label free技术尤为适用。该技术在各类文献中应用已经非常广泛。 | 技术路线 |...
Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import.Nat Commun.2018 Sep. 本文通过SiRNA技术在人细胞中构建Sec61和TRAP敲除细胞模型,通过Label free 蛋白组学技术分析差异蛋白,寻找TRAP依赖性多肽,并在细胞模型和CDG病人细胞中进行WB和qPCR验证。结果...
Biopharmaceutical Perspectives Biopharmaceuticals and Protein Analysis Cannabis Analysis Capillary Electrophoresis Chiral Chromatography ChromAcademy Corporate Profiles Data Acquisition, Handling, and Archiving Data Analysis, Statistics, and Chemometrics Dietary Supplements Analysis ...
We have launched 4D label-free proteomics services to help research in microsample proteomics and larger cohorts of clinical samples. These services are accompanied by a high-quality full after-sales service.
2023年7月，郑州大学第一附属医院研究团队在期刊Journal of Proteome Research 上发表了题为“Integration of Metabolomics, Lipidomics, and Proteomics Reveals the Metabolic Characterization of Nonalcoholic Steatohepatitis”的文章。该研究团队利用Label free蛋白组学结合代谢组学、脂质组学技术，表征NASH患者的代谢谱。中...
2023年7月,郑州大学第一附属医院研究团队在期刊Journal of Proteome Research上发表了题为“Integration of Metabolomics, Lipidomics, and Proteomics Reveals the Metabolic Characterization of Nonalcoholic Steatohepatitis”的文章。该研究团队利用Label free蛋白组学结合代谢组学、脂质组学技术,表征NASH患者的代谢谱。中科...
2023年7月,郑州大学第一附属医院研究团队在期刊Journal of Proteome Research上发表了题为“Integration of Metabolomics, Lipidomics, and Proteomics Reveals the Metabolic Characterization of Nonalcoholic Steatohepatitis”的文章。该研究团队利用Label free蛋白组学结合代谢组学、脂质组学技术,表征NASH患者的代谢谱。中科...
定量蛋白质组学(Quantitative Proteomics)是蛋白质组学研究领域最重要的应用之一。通过定量蛋白质组学技术,可以对样品之间的蛋白质水平的变化进行定量比较。由此获得蛋白质水平上关于疾病发生,细胞代谢等过程的整体而全面的认识。 产品介绍 Label-free非标记定量技术是一种不依赖于同位素标记的蛋白质定量技术,该技术通过...
Large-Scale Label-Free Comparative Proteomics Analysis of Polo-Like Kinase 1 Inhibition via the Small-Molecule Inhibitor BI 6727 (Volasertib) in BRAFV600E Mutant Melanoma Cells. J Proteome Res. 2014 Jun 9. 【技术特点对用户带来的好处】-- 非标(Label free)蛋白质组学技术 【典型应用举例】-- ...