Fig2. CreERT2-mediated recombination in Small intestine of Prox1CreERT2/+;Rosa26tdTomato/+mouse. Fig3. Detection of tdTomato(red) in various tissues of Prox1CreERT2/+;Rosa26tdTomato/+mice. Labeling was also observed in pituitary gland, retina, heart, thymus, stomach, lung, submandibular...
Comparison of the Prox1-Cre-tdTomato mouse with the Prox1-Cre-tdRFP mouse.Roberta, BianchiAlvaro, TeijeiraSteven, T. ProulxAilsa, J. ChristiansenCatharina, D. SeidelThomas, RülickeTaija, MäkinenRené, HägerlingCornelia, HalinMichael, Detmar...
The Prox1-Cre-tdTomato reporter mouse thus shows great potential for lymphatic research.RobertaBianchiAlvaroTeijeiraSteven TProulxAilsa JChristiansenCatharina DSeidelPloS oneBianchi R, Teijeira A, Proulx ST, Christiansen AJ, Seidel CD, Rulicke T, et al. A transgenic Prox1-Cre-tdTomato reporter...
然而,只有Prox1、Onecut2和Klf15在肝脏中终身表达(图1b、f)。为了测试它们是否促进肝细胞的同一性,我们在小鼠胚胎成纤维细胞(mouse embryonic fibroblasts, MEFs)向肝细胞重编程过程中过表达它们(图1g)。通过TJP1蛋白表达测量,PROX1使肝细胞样细胞诱导增加了10倍以上,每个细胞白蛋白分泌增加了17倍,并促进了肝细胞...
[27] Prox1-CreERT2 小鼠模型来自于Taija Makinen 实验室 。靶向Tie2 的条件性基因敲除小鼠 Flox [18] 模型(Tie2 )为本课题组在南京大学模式动物研究所完成 。本研究涉及的所有动物实 90 验均按照苏州大学实验动物中心规定进行操作。 1.1.2 抗体 本研究所用抗体包括:Rat-anti-mouse PECAM- 1 (553370 )购...
The Prox1-Cre-tdTomato mouse is a valid tool for intravital microscopy of DC migration.Roberta BianchiAlvaro TeijeiraSteven T. ProulxAilsa J. ChristiansenCatharina D. SeidelThomas RülickeTaija MäkinenRené HägerlingCornelia HalinMichael Detmar...
mouse phenotypes. W.M. generated and providedProx1+/GFP-Creembryos. J.K. performed ChIP, ChIP–seq and RNA-seq experiments and assessed mouse lymphatic phenotypes. J.K., J.T., L.A.-M. and A.W.S. analysed the bioinformatic data. S.T. advised on embryonic haematopoiesis assays. J.K...
(Fig.1b,f). To test whether they promote hepatocyte identity, we overexpressed them in mouse embryonic fibroblasts (MEFs) during reprogramming toward hepatocytes20(Fig.1g). PROX1 increased hepatocyte-like cell induction greater than tenfold, as measured by TJP1 protein expression, elevated albumin...
Since PROX1 is also expressed in the hepatocytes of the adult liver55, it would be interesting to assess whether in our Prox1-CRE-ERT2 transgenic mouse model starvation further aggravates the lym- phangiogenic defects observed after loss of Atg5 in lymphatic EC and hepatocytes. Considering that...
The inherent fluorescence of blood and lymphatic vessels allows for direct visualization of blood and lymphatic vessels in various organs via confocal and two-photon microscopy and the formation, branching, and regression of both vessel types in the same live mouse cornea throughout an experimental ...