[2] Xue W, Chen S, Yin H, et al. CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature. 2014;514(7522):380-384. doi:10.1038/nature13589 [3] Li Y, Liu Z, Han X, et al. Dynamics of...
Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes. Lab Anim Res. 2018; 34(4): 147–159. 5. McLellan MA, Rosenthal NA, Pinto AR. Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations. Curr Protoc Mouse Biol. 2017;7(1)...
“Rescue-like behavior in a bystander mouse toward anesthetized conspecifics promotes arousal via a tongue-brain connection.” Science advances vol. 11,4 (2025): eadq3874. doi:10.1126/sciadv.adq3874 使用我司病毒: rAAV2/Retro-hSyn-Cre-WPRE-hGH pA rAAV2/9-DIO-hM4D(Gi)-mCherry rAAV2/9-...
4小结大家在实际实验过程中要进行根据实验目的和基因表达特性选择合适的诱导时间，过早或过晚的诱导都可能影响实验结果。在使用这些药物时，需要综合考虑实验需求、药物特性以及实验动物的生理状态等因素，以确保实验的成功和结果的可靠。参考文献：[1] Kim H, et al. Mouse Cre-LoxP system: general principles to d...
[1] Kim, H., Yonsei University College of Medicine, Seoul, Kim, M., Yonsei University College of Medicine, Seoul, Im, S.K., Yonsei University College of Medicine, Seoul, et al. Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes[J]. Laboratory ...
[1] Kim H, et al. Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes. Lab Anim Res. 2018 Dec;34(4):147-159. [2] Indra AK, et al. Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase...
即先把目的基因(gene of interest)沉默掉(在前方加一个终止子),通过把stop拿掉而条件性激活。 (3) Cre构建报告小鼠 reporter mouse。比如,想让Gli1+ cells 都绿的发慌。首先介绍小鼠基因组内两个外源性基因的安全敲除位点:Rosa26和H11。Rosa26是小鼠6号染色体上由3个外显子构成的非编码基因,易插入外源基因,易...
14.Zhang, Y., et al. (1996). Inducible site-directed recombination in mouse embryonic stem cells. Nucleic Acids Research, 24(3), 543-548. doi:10.1093/nar/24.3.543 15.Indra, A. K., et al. (1999). Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: co...
1.H, K., et al., - Mouse Cre-LoxP system: general principles to determine tissue-specific roles of. - Lab Anim Res. 2018 Dec;34(4):147-159. doi: 10.5625/lar.2018.34.4.147. Epub 2018, (- 1738-6055 (Print)): p. - 147-159. ...
[3] BLA DBS improves anxiety and fear by correcting weakened synaptic transmission from BLA to adBNST and CeL in a mouse model of foot shock 2024,《Cell Reports》,IF: 8.8 [4] A microglial activation cascade across cortical regio...