Multiplex PCRMultiple antimicrobial resistancesPPNG (penicillinase-producing N. gonorrhoeaeTRNG (tetracycline-resistant N. gonorrhoeaeCRNG (ciprofloxacin-resistant N. gonorrhoeaeGonorrhea is a continuing problem worldwide particularly in terms of the spread of multiple drug resistance. We have successfully ...
blood samples from febrile oncohaematological patients were tested using a novel commercial real-time PCR assay (LightCycler SeptiFast; Roche Molecular Systems... N Mancini,D Clerici,R Diotti,... - 《Journal of Medical Microbiology》 被引量: 265发表: 2008年 Multiplex PCR System for Rapid Detect...
摘要: New multiplex PCR-based protocol allowing indirect diagnosis of FSHD on single cells: can PGD be offered despite high risk of recombination?European Journal of Human Genetics 18, ...关键词: FSHD, single cell, multiplex PCR, indirect diagnosis, PGD, recombination ...
The aim of this study was to develop a rapid multiplex PCR for the simultaneous detection of HPV and C. trachomatis in ThinPrep liquid cytology samples. Two multiplexes were optimized. (A) For the detection of C. trachomatis using primers for the cryptic plasmid and for a chromosomal gene ...
The PCR performed in a 25 µl reaction mixture containing 10 ng DNA, 1x ThermoPol® buffer (with 2 mM MgCl2 or MgSO4), 20-200 nM of primer (for multiplex PCR, primer combinations – maximum 0.5 µM is total concentration), 0.2 mM dNTPs, 1 U Taq DNA polymerase and (optionally)...
Genotyping PCR design Timing: 30 min This section is the detailed protocol for designing a pair of primers containing CRISPR target sequence. Note: The distance difference from the primers to the sgRNA sequences should be more than 50 bp. Design of single guide RNA oligos Timing: 1–2 h ...
3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA. 4. Procedures are described for identifying the sex of...
Here we extend the utility of a previously designed multiplex PCR by designing a primer set specific to parasites of the subgenus Haemoproteus (genus: Haemoproteus). The updated one-step multiplex PCR protocol we describe here allows for the detection of the genera Plasmodium and Leucocytozoon ...
A simple and reliable protocol for the detection of apple stem grooving virus by RT-PCR and in a multiplex PCR assay. Primers were identified which amplify specifically a 499 bp fragment in the coat protein coding region of apple stem grooving virus (ASGV) genome. These pr... D James -...
surface and intracellular protein markers can also be stained with fluorescently labeled antibodies for simultaneous protein and mRNA measurement. Moreover, a semiautomated, single-tube version of the protocol can be performed with a commercially available cell-wash device that reduces cell loss, operato...