Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in ...
Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C) The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment The Proteinase K, Lyophilized powder can ...
Protease-activated receptors (PAR) are present in the urinary bladder, and their expression is altered in response to inflammation. PARs are a unique class of G protein-coupled that carry their own ligands, which remain cryptic until unmasked by proteoly
resuspending the insoluble proteins in an aqueous solution and adding PEG to the solution, wherein the temperature and pH of the solution and concentration of PEG is selected to precipitate.alpha.-2 proteins without precipitating alpha-1-PI; recovering the supernatant from the PEG precipitation and...
The “binding constant” K is defined as the reciprocal of Kd. The measurement of the thermal unfolding of NS2B/NS3prot with (150 μM) and in the absence of SUV was performed in the 20–85 °C temperature range with increments of 5 °C. The native protein fraction (fN) was ...
Proteinase K Usage And Synthesis Description Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has...
Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C) The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment ...
Proteinase K may be added either at the initial steps, directly to the cell/tissue homogenate, where its role is to facilitate sample digestion (for example, when working with highly fibrous tissue) or, as in our case (Protocol_P, #80224), to the lysate devoid of tissue debris. In such...