Proteinase K Chemical Properties,Uses,Production Description Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. ...
英文名称:Proteinase K, Tritirachium album 分子式:null 产地:上海 品牌:PERFEMIKER 包装规格:100mg 货号:539480-100MG CAS编号:39450-01-6 别名:蛋白酶K,Tritirachium相册 References ReferencesWatazu, Y., et al. 1993.J. Lab. Clin. Anal.7,81. ...
Compared to WTPK, TLPK shows over 20°C more labile to heat inactivation. The melting temperature (Tm) of TLPK is also around 25°C lower than that of WTPK, decreasing from 75.9°C to 50.9°C. TLPK greatly outperforms a broad specificity protease isolated from an arctic marine ...
20mg/ml蛋白酶K溶液/Proteinase K solution 产品信息 DescriptionFrequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product. Application Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.Removal endotoxins bound to cationic ...
蛋白酶K MERCK相关资料如下: 539480 Proteinase K, Tritirachium album ( Proteinase K, Tritirachium album ) Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucle...
Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C) The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment The Proteinase K, Lyophilized powder can ...
Mechanism of thermal inactivation of plasmin with developing covalent disulphide interaction between molecule of plasmin and serum proteins (mostly ß-laktoglobulin) is described. Thermosensitive inhibitors of plasminogen activators and inhibitors of plasmin are inactivated by short pasteurisation and ...
In the study by García-Risco, Olano, Ramos, and López-Fandiño (2000) it was reported that plasmin in bovine milk resists HPP treatments at 400 MPa for 15 min, but an increase of temperature produced a strong decrease of its activity undergoing 86.5% inactivation at 60°C. ...
4. 4. Rapid inactivation caused by temperature change and dilution was completely prevented in the presence of substrate proteins. 5. 5. The bonds cleaved by the enzyme were Leu15-Tyr16 and Tyr16-Leu17 of insulin β-chain and Phe(NO2)-Phe of the synthetic substrate. 展开 ...
Protease-activated receptors (PAR) are present in the urinary bladder, and their expression is altered in response to inflammation. PARs are a unique class of G protein-coupled that carry their own ligands, which remain cryptic until unmasked by proteoly