Description Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, ...
Proteinase K remains active: • Over a wide pH range—optimal activity between 6.5 and 9.5 • Under denaturing conditions—e.g., in the presence of SDS or urea • In the presence of metal chelating agents—e.g., EDTA • At comparatively high temperatures—optimum digestion temperature ...
Temperature characteristic The effective activity temperature is 37-70°C, and the enzyme activity at 65°C is twice that at 25°C. PH characteristics 4.0-12.0, the optimum range is ph7.5-11.5 Preservation Conditions It is recommended to store at -20°C to ensure the...
• At comparatively high temperatures (optimum digestion temperature is 65°C) Applications Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections forin situhybridization. Performance and quality testing ...
The proteolytic activities and some important characteristics such as optimum pH and temperature of the P-hNFs were also evaluated by comparison with free proteinase K. Optimum pH values of free proteinase K and P-hNFs were determined as pH 10 and pH 11, respectively. Optimum temperatures ...
Temperature characteristic The effective activity temperature is 37-70°C, and the enzyme activity at 65°C is twice that at 25°C. PH characteristics 4.0-12.0, the optimum range is ph7.5-11.5 Preservation Conditions It is recommended to store at -20°C to ensure the stability of activity...
pH optimum (alkaline, neutral, and acid proteinases) and temperature range • specific amino acids that are central for hydrolytic activity (aspartic, cysteine, serine) and/or the requirement for metal ions (metalloproteinases) Table 1. Characteristics of some animal, microbial, and plant proteinase...
pH value optimum 7.5 - 12.0 Solubility 50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 EC number 3.4.21.14 CAS number 39450-01-6 Store and ship information Storage +2°C to +8°C Ship Ambient Temperature Only Irritant **标示 S Phrase S: 22-24-26-36/37 Do not breathe...
Proteinase K remains active:• Over a wide pH range—optimal activity between 6.5 and 9.5• Under denaturing conditions—e.g., in the presence of SDS or urea• In the presence of metal chelating agents—e.g., EDTA• At comparatively high temperatures—optimum digestion temperature is 65...
The temperature optimum of the insolubilized enzyme showed a dramatic upward shift to 60–70°C from the native enzyme's 50°C. The insolubilized enzyme was also relatively more stable over a wide temperature range of 35–80°C and from pH 4·0 to 11·0. The activity of the native ...