Protein translocase subunit SecY (Rv0732) has been selected since it is a highly ranked potential drug target without solved three-dimensional structure. In silico structural analysis has been carried out to get descriptive three-dimensional structure. The models were generated using crystal structure ...
Indeed, FtsH can degrade the major translocase subunit SecY46 and this activity can even lead to a lethal defect in protein secretion if not properly regulated47. Phenotypically, ftsH mutants were similar to FY mutants in that they displayed a small colony size on plates (Supplementary Fig. 7a...
Sec systems are versatile translocases capable of transporting soluble protein completely across the bilayer, of integrating multispanning membrane proteins, and a combination of both for those proteins that contain INTRODUCTION Studies of the thylakoid Sec system benefited from the extensive knowledge of ...
SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycl... A Economou,W Wickner - 《Cell》 被引量: 959发表: 1994年 The purified E. coli integral membrane protein SecY/E is sufficient for...
The preprotein translocase of the outer mitochondrial membrane (Tom) is a multisubunit machinery containing receptors and a general import pore (GIP). We h... SandravanWp,M Ryan,KrsnH,... - 《Nature》 被引量: 393发表: 2000年 The SecA and SecY subunits of translocase are the nearest ne...
SecA, the ATPase motor of post-translational export, 'proof-reads' its substrates, which are possibly already at the ribosome, and assembles with SecYEG into the translocase holoenzyme. SecA is allosterically activated by amino-terminal signals that are present on exported proteins and uses its ...
It is imported via the TOM and TIM23 protein translocases into the mitochondrial matrix were it is processed. Processing of the precursor by MPP and MIP (cleavage sites are indicated by green arrows) leads to the mature-sized apo-protein. The apo-protein binds a 2Fe2S cluster that is ...
TolC is an OMP that homo-trimerizes to form a large outer-membrane export tunnel that interacts with inner-membrane translocases73,74. The catalytic β-barrel domain of BamA binds substrates along the β-barrel seam during OMP folding, and in this process, the N-terminal of the β-barrel ...
(sec61α, sec61β and sec61γ in eukaryotes). The cytoplasmic SecA subunit hydrolyzes ATP to drive translocation. Proteins may be targeted to the translocase via two routes. Membrane proteins and proteins with very hydrophobic signal sequences are translocated co-translationally; the signal sequence...
ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase. Cell 64, 927–939 (1991) Article CAS PubMed Google Scholar Irihimovitch, V. & Eichler, J. Post-translational secretion of fusion proteins in the halophilic archaea Haloferax volcanii . J. Biol. ...