Protein translocase subunit SecY (Rv0732) has been selected since it is a highly ranked potential drug target without solved three-dimensional structure. In silico structural analysis has been carried out to get descriptive three-dimensional structure. The models were generated using crystal structure ...
Subunit interactions in the Escherichia coli protein translocase: SecE and SecG associate independently with SecYdoi:10.1016/s0014-5793(97)00376-1Takayuki HommaTohru YoshihisaKoreaki ItoHomma, T., Yoshihisa, T., and Ito, K. (1997) Subunit interac- tions in the Escherichia coli protein ...
This encodes a functional homologous to of , identified as a subunit of the preprotein translocase complex. Similarity of the and topology, predicted from sequence information, has been confirmed experimentally through -fusion analysis in . , expressed in an mutant, substituted for the defective ...
Sec systems are versatile translocases capable of transporting soluble protein completely across the bilayer, of integrating multispanning membrane proteins, and a combination of both for those proteins that contain INTRODUCTION Studies of the thylakoid Sec system benefited from the extensive knowledge of ...
SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycl... A Economou,W Wickner - 《Cell》 被引量: 959发表: 1994年 Structure and function of a membrane component SecDF that enhances protein...
T. Subunit dynamics in Escherichia coli preprotein translocase. Proc. Natl Acad. Sci. USA 91, 4703–4707 (1994). Article CAS ADS PubMed PubMed Central Google Scholar Breyton, C., Haase, W., Rapoport, T. A., Kuhlbrandt, W. & Collinson, I. Three-dimensional structure of the ...
TolC is an OMP that homo-trimerizes to form a large outer-membrane export tunnel that interacts with inner-membrane translocases73,74. The catalytic β-barrel domain of BamA binds substrates along the β-barrel seam during OMP folding, and in this process, the N-terminal of the β-barrel ...
The protein components in the inner membrane that are required for the operation of the Sec pathway are shown. ΔpH/ΔΨ, proton-motive force; SP, signal peptide; SPase, signal peptidase. SecAp is part of a multisubunit preprotein translocase complex in the plasma membrane together with Sec...
SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins. Unlike the SecD subunit of the pre-protein translocase of Escherichia coli, SecDF of B. subtilis was not required for the release of a mature secretory... A Bolhuis,CP Broekhuizen,A Sorokin...
SecA, the ATPase motor of post-translational export, 'proof-reads' its substrates, which are possibly already at the ribosome, and assembles with SecYEG into the translocase holoenzyme. SecA is allosterically activated by amino-terminal signals that are present on exported proteins and uses its ...