Preprotein translocase, the membrane transporter for secretory proteins, is a processive enzyme. It comprises the membrane proteins SecYEG(DFYajC) and the peripheral ATPase SecA, which acts as a motor subunit. Translocase subunits form dynamic complexes in the lipid bilayer and build an aqueous ...
The preprotein translocase of the outer mitochondrial membrane (Tom) is a multisubunit machinery containing receptors and a general import pore (GIP). We h... SandravanWp,M Ryan,KrsnH,... - 《Nature》 被引量: 393发表: 2000年 The SecA and SecY subunits of translocase are the nearest ne...
SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycl... A Economou,W Wickner - 《Cell》 被引量: 959发表: 1994年 The purified E. coli integral membrane protein SecY/E is sufficient for...
The YidC–Oxa1–Alb3 preprotein translocases play a vital role in membrane insertion of proteins in eukaryotes and bacteria. In a recent study we observed that Rv3921c, which encodes putative YidC translocase in Mycobacterium tuberculosis (Mtb), is essential for in vitro growth of bacteria....
Translocase is a complex nanomachine that moves processively along its aminoacyl polymeric substrates effectively pumping them to the periplasmic space. The salient features of this process are: (a) a membrane-embedded "clamp" formed by the trimeric SecYEG protein, (b) a "motor" provided by ...
Most bacterial secretory proteins destined beyond the plasma membrane are secreted post-translationally by the Sec translocase. In the first step of translocation, preproteins are targeted for binding to their 2-site receptor SecA, the peripheral ATPase subunit of the translocase. We now reveal that...
Protein export across the bacterial inner membrane is catalyzed by preprotein translocase, a multisubunit enzyme composed of integral and peripheral membrane domains (1). The essential or “core” subunits of this complex, identified by genetic and biochemical studies, are the membrane-embedded hetero...
Mitochondrial YME1L1 governs unoccupied protein translocase channels Meng-Chieh Hsu Hiroki Kinefuchi Richard J. Youle Nature Cell Biology(2025) Mitochondrial protein import stress Nikolaus Pfanner Fabian den Brave Thomas Becker Nature Cell Biology(2025) ...
The preprotein translocase of Escherichia coli is a multisubunit enzyme with two domains, the peripheral membrane protein SecA and the membrane-embedded SecY/E protein. SecY/E has been isolated as a complex of three polypeptides, SecY, SecE, and band 1. We now present four lines of ...
SecA is the dissociable ATPase subunit of the Escherichia coli preprotein translocase, and cycles in a nucleotide-modulated manner between the cytosol and the membrane. Overproduction of the integral subunits of the translocase, the SecY, SecE and SecG polypeptides, results in an increased level ...