Protein Thermal Shift™ 软件的开发是w为了分析从 Applied Biosystems® 实时荧光定量 PCR 仪文件中直接得出的蛋白熔解荧光读数。不同的蛋白具有不同的 Protein Thermal Shift 表达谱,各自具有独特的熔解曲线形状、斜率、信噪比和温度熔解范围。Protein Thermal Shift™ 软件可通过...
Protein Thermal Shift Control Ligand is provided at a concentration of 100 mM. Guidance on how to set up the reaction components for the protein melt reaction can be found on page 36 of the user guide: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025600_Protein_Thermal_Shift_...
Protein Thermal Shift™ (PTS) reagents enable protein melt assays that can be used as an efficient screening tool for measuring protein thermal stability, identifying suitable buffer conditions, and measuring protein–ligand interactions. The assay is very easy to set up and run, with typical time...
The Protein Thermal ShiftTM (PTS) Assay is anexcellent high-throughput screening methodwhich enables researchers to rapidly monitorprotein thermo-stability and to identify optimalconditions that favor protein stability, includingthe investigation of protein-ligand interactionsand mutations in protein sequenc...
The Tm values are determined by differential scanning fluorimetry (DSF) method using the Protein Thermal Shift Dye Kit (Thermo Fisher). One microlitter of the SSYPRO Orange dye (SUPELCO, USA) is added to 49 μL lysis buffer (25 mM Tris-HCl,...
APPLICATION NOTE Protein Thermal Shift technology Protein Thermal Shift technology Optimizing buffer conditions and high-throughput screening of ligand-protein binding The Applied Biosystems™ Protein Thermal Shift™ assay measures protein thermal stability using a fluorescent protein–binding dye. Protein ...
Thermal stability of the nanoparticles was determined using a protein thermal shift assay kit (Thermofisher) in 384-well PCR plates. About 20 μl of reaction mixture containing 12.5 μl of sample, 2.5 μl of Protein thermal shift dye (8X), and 5 μl of Protein thermal shift bu...
We examined the thermolability of a mutant form of MMACHC that is responsive to OHCbl but not to CNCbl in patients. Our previous finding that recombinant MMACHC-R161Q binds OHCbl with wild-type affinity provided a facile explanation for why patients respond clinically to OHCbl. We had shown...
Recently, cellular thermal shift assay has been recognized as a valuable tool for validating drug targets based on the principle that binding of the test compound at the functional sites of the target proteins could prevent the thermal precipitation of the target proteins [28,29]. In the present...
实验方案:快速参考指南:Protein Thermal Shift 实验方案:Quick Reference Card: Protein Thermal Shift™ Studies: Buffer Screening 实验方案:Quick Reference Card: Protein Thermal Shift™ Studies: Ligand Screening 技术说明:Protein Thermal Shift染料:热稳定性...