1a). Thus, we hypothesized that these 3′UTR piRNA precursors may be controlled by the transcription factor A-MYB, which also promotes the synthesis of pachytene piRNA precursors8. Chromatin immunoprecipitation sequencing (ChIP-seq) revealed that A-MYB-binding sites are far from the 3′UTRs of...
Recent breakthroughs in AI coupled with the rapid accumulation of protein sequence and structure data have radically transformed computational protein design. New methods promise to escape the constraints of natural and laboratory evolution, accelerating the generation of proteins for applications in biotechno...
Milk proteins and milk protein-derived peptides have been widely studied for their health enhancing properties. This chapter presents the updated scientific knowledge on the bioactive properties of milk protein-derived peptides. The different bioactive p
Cell free synthesis as a new manufacturing platform? Historically biological medicines have been produced by live cell fermentations where, as the name suggests, live cells are used to produce the product. In this model the reagent (cell) generation step is intricately linked to the product genera...
Proposed activity of cyclin D3 in IAV budding. (a) In normal cells, cyclin D3 is localized to the nucleus where it regulates cell cycle; (b) during IAV infection, cyclin D3 interacts with viral M2 to disrupt M1-M2 binding; (c) in the absence of cyclin D3, more infectious progeny ...
Stability of proteins in cells was monitored in a pulse-chase labeling and immunoprecipitation experiment (Breitschopf et al., 1998) or after inhibition of protein synthesis by cycloheximide as described (Gross-Mesilaty et al., 1998). In all experiments, cells were lysed in a buffer containing...
@article{rocklin2017, title={Global analysis of protein folding using massively parallel design, synthesis, and testing}, author={Rocklin, Gabriel J and Chidyausiku, Tamuka M and Goreshnik, Inna and Ford, Alex and Houliston, Scott and Lemak, Alexander and Carter, Lauren and Ravichandran, Ras...
Phosphorylation of TSC2 by PKC-δ reveals a novel signaling pathway that couples protein synthesis to mTORC1 activity. Phosphorylation of TSC2 by PKC-δ reveals a novel signaling pathway that couples protein synthesis to mTORC1 activity.doi:10.1007/s11010-019-03498-8PKC... Jun,Zhan,Raghu,......
A universal gain-of-function approach for selective and temporal control of protein activity in living systems is crucial to understanding dynamic cellular processes. Here we report development of a computationally aided and genetically encoded proximal decaging (hereafter, CAGE-prox) strategy that enables...
JUN proteins can both homo- and hetero-dimerize, whereas FOS proteins only form hetero-dimers, which, particularly for JUN/FOS, are more stable and have greater transcriptional activity [9,10]. Intriguingly, c-FOS and FOSB contain transcriptional activation domains which are lacking in FRA-1 and...