DNA Structure and Protein Synthesis Lab ActivityUnderstanding DNA
there is a growing need for designed proteins with optimized characteristics, such as elevated thermostability, higher binding affinity, or increased catalytic activity. Natural proteins are often used as starting points for the development of useful proteins, which can then be engineered as high...
Two peptide arrays were generated (Peptide Synthesis Laboratory, CR UK LRI, London): (1) interaction array: 82 different 20-mer peptides each shifted by 1 aa and covering the whole sequence of CENP-CFBDwere spotted onto a cellulose membrane; (2) substitution array: each and every residue ...
Recent breakthroughs in AI coupled with the rapid accumulation of protein sequence and structure data have radically transformed computational protein design. New methods promise to escape the constraints of natural and laboratory evolution, accelerating the generation of proteins for applications in biotechno...
In particular, local dendritic protein synthesis represents a plausible mechanism by which the morphology of individual spines is modulated in a synapse-specific manner [85]. Several lines of evidence support the notion that dendritic protein synthesis is involved in activity-dependent spine morphogenesis...
In CCN1-3, the TSP1 domain (Fig.1, purple) is wedged between the vWC and the CK domains, with the CK domain wrapped around it and restricting its position. Thus, the CK domain may confer steric hindrance that could affect activity of the TSP1 domain. Coordinated activities may also res...
@article{rocklin2017, title={Global analysis of protein folding using massively parallel design, synthesis, and testing}, author={Rocklin, Gabriel J and Chidyausiku, Tamuka M and Goreshnik, Inna and Ford, Alex and Houliston, Scott and Lemak, Alexander and Carter, Lauren and Ravichandran, Ras...
Sp1 was shown to affect ECP synthesis by binding to the promoter region of the gene. Also, the intronic SNP c.-38A>C has been shown to relate to the ECP content of eosinophils. Thus, several parts of the ECP gene seem to affect ECP production. In a Japanese population, a promoter ...
et al. Programmable eukaryotic protein synthesis with RNA sensors by harnessing ADAR. Nat Biotechnol 41, 698–707 (2023). https://doi.org/10.1038/s41587-022-01534-5 Download citation Received23 May 2022 Accepted28 September 2022 Published27 October 2022 Issue DateMay 2023 DOIhttps://doi.org/...
The model we use for protein synthesis, folding, and function has been shown to accurately predict experimentally measured changes in enzyme specific activities32, indicating it reasonably describes protein structure-function relationships. As an additional test, here we examine if the model is able to...