The activation of the unfolded protein response (UPR) is closely linked to the pathogenesis of renal injuries. However, the role of XBP1, a crucial regulator of adaptive UPR, remains unclear during the transition from acute kidney injury (AKI) to chronic
Many unannotated microproteins and alternative proteins (alt-proteins) are coencoded with canonical proteins, but few of their functions are known. Motivated by the hypothesis that alt-proteins undergoing regulated synthesis could play important cellular
we provide a conceptually different design of a cell with two orthogonal protein synthesis systems, where Ribo-T produces the proteome, while the dissociable ribosome is committed to the translation of a specific mRNA. The utility of this system is illustrated by generating a comprehensive collection...
A diagram of the selection process and output analysis is presented in Fig. 1. Plasmid DNA was purified from polyclonal yeast from the PtdIns(4,5) and PtdIns(3,4,5) round three selection outputs and used as a template for in vitro transcription reactions to generate biotin-labeled RNA ...
Analysis of ORF21 (HmaS) shows the enzyme is a novel non-heme iron dioxygenase that catalyzes the synthesis of L-p-hydroxymandelate from p-hydroxyphenylpyruvate. The L-p-hydroxymandelate generated by HmaS is subsequently converted to L-p-hydroxyphenylglycine via ORF22 (Hmo) catalyzed conversion ...
Gallium-68-labeled chelator-linked FAP inhibitors (FAPIs) have been successfully applied to positron emission tomography (PET) imaging of various tumor types. To broaden the spectrum of applicable PET tracers for extended imaging studies of FAP-dependent diseases, we herein report the radiosynthesis ...
The model we use for protein synthesis, folding, and function has been shown to accurately predict experimentally measured changes in enzyme specific activities32, indicating it reasonably describes protein structure-function relationships. As an additional test, here we examine if the model is able to...
(arbitrary units, A.U.) of the indicated proteins in the TZ. Scale bar, 1 μm.eTEM imaging showing the longitudinal sections of TCTN1–HA transition zones labeled with anti-HA (10-nm gold, red arrowheads). Scale bar, 0.1 μm.fSchematic of the localization of TCTN1, CEP290, ...
Preliminary experiments established the printing conditions with fluorescently labeled OX68 mAb. Of several types of slides tested, the epoxy-coated ones were the best in terms of spot morphology, cost and reproducibility and were used in all subsequent experiments. Most array designs were performed ...
1 (see "Methods" for details of the synthesis). CID797718 is a byproduct of CID755673 synthesis, and has 10-fold less inhibitory activity toward PKD than the parental compound [20]. The design of the CID755673 analogs was based on initial structure-activity relationship (SAR) analysis ...