cDNA synthesis was carried out using 1 μg RNA and iScript Reverse Transcription Supermix (Bio-Rad Cat #1708841), and RT-qPCR was performed using Advanced Universal SyBr Green Supermix (Bio-Rad Cat #725271) on the CFX384 detection system (Bio-Rad). RT-qPCR was carried out in technica...
performed the chemical synthesis; S.-Q.L. helped with data analysis and interpretation, revised the manuscript; K.Y. helped with data analysis and interpretation. Corresponding authors Correspondence to Chun Tang, Meng-Qiu Dong or Xiaoguang Lei. Ethics declarations Competing interests The authors ...
pH-dependent regulation of electron transport and ATP synthesis in chloroplasts. Photosynth. Res. 116, 511–534.Over the years, various spectroscopic techniques have been used to study the EET and charge separation in PSII (see reviews by
This acidic portion of proMBP may serve to protect cellular structures from its cytotoxic activities during synthesis and packaging. The larger proMBP, however, has been identified in immature bone marrow cells. proMBP has also been found in placental cells in complex with the metalloproteinase ...
MCC/eisosomes are important for cell wall synthesis and organisation as well as the recruitment of proteins to the plasma membrane [107]. We speculate that these fungal-specific microdomains also have a role in EV biogenesis from C. albicans, and potentially other fungi, based on the role of...
[7,8]. Diverse proteins, such as transcription factors, histones, and cell cycle regulators, need to be transported into the nucleus through the NPC after their synthesis, which necessitates the presence of a nuclear localization signal (NLS) on these cargo proteins [9]. The NLS is ...
Models of drug–target binding.aSchematic diagram of a one-barrier drug–target binding free energy profile. A one-step model with one free energy barrier is used to derive the experimental rate constants. The figure and equations show how the steady-state rate constants relate to the free ene...
a, Plasmid DNA containing an antibiotic-resistance (ABR) gene and a gene of interest (GOI) flanked by LHA and RHA. A double-crossover insertsABRandGOIinto the target locus, replacing genomic DNA.b, Diagram of primer pairs for PCR genotyping. Amplification of LHA and RHA includes one priming...
which controls the translational efficiency. These two proteins are necessary for attenuation of protein synthesis in response to osmotic stress [50], and the interaction among them was observed in the constructed subnetwork. Several proteins influence nuclear residence of Hog1p, such as the tyrosine ...
[6, 7], iv) cysteine to improve glutathione status and muscle protein synthesis, v) high concentrations of branched chain amino acids, and vi) minor bioactive components such as immuno-globulins, growth factors, and anti-microbial proteins and peptides to assist in effective gut function [6,...