aDiagram of the gene structure ofTCTN1(Cre03.g181450) with theaphVIIIDNA insertional site.bDiagram of the domain structure of the TCTN1 protein. The TCTN1 protein (679 aa) contains the DUF1619 domain (78–418 aa) with unknown function. The red arrowhead marks the insertional site.cDIC ...
To generate plasmids for exogenous expression of PPM5C, thePPM5Ccoding region was amplified by PCR from cDNA prepared from total RNA using the Maxima H Minus First Strand cDNA Synthesis Kit (Fisher Scientific). Total RNA was isolated from ∆ku80 parasites using the RNeasy Plus Mini Kit (QIA...
Two peptide arrays were generated (Peptide Synthesis Laboratory, CR UK LRI, London): (1) interaction array: 82 different 20-mer peptides each shifted by 1 aa and covering the whole sequence of CENP-CFBDwere spotted onto a cellulose membrane; (2) substitution array: each and every residue ...
The synthesis of Zein nanoparticles (NPs) using conventional methods, such as emulsion solvent diffusion and emulsion solvent evaporation, is often unreliable in replicating particle size and polydispersity between batch-to-batch syntheses. We have systematically examined the parameters for reproducibly synth...
The commercial production of mAbs and other biotherapeutic products is currently based on synthesis in 5 to 20 m3 bioreactor tanks with mammalian cells by shaking suspension culture operated in a fed-batch or perfusion mode [19,20,21]. The commercial production of mAbs in stirred tanks faces ...