Structure and Mechanism of Acetylation by the N-Terminal Dual Enzyme NatA/Naa50 Complex. Structure 27, 1057–1070.e4 (2019). Article CAS PubMed PubMed Central Google Scholar Layer, D. et al. Structural basis of Naa20 activity towards a canonical NatB substrate. Commun. Biol. 4, 1–12...
acetylation, methylation, glycosylation, nitrosylation, lipidation, ubiquitylation, and neddylation among others, affect many aspects of normal cell biology, and are essential for the maintenance of the homeostasis of a cell.1,2Specifically, neddylation is an ubiquitylation-like (UBL) modifications.3NEDD...
First, genome comparison indicates that thermophilic organisms (especially hyperthermophiles) have a reduced complement of chaperones relative to the other domains of life (Laksanalamai, Whitehead, & Robb, 2004). This property implies that the remaining chaperones each exhibit folding activity for a ...
The proteins were reduced (2 mM TCEP, 1 h) and alkylated (40 mM iodoacetamide, 30 min in the dark) before digestion with 1 μg trypsin (Promega) for 18 h at 37°C. Peptides were then extracted from the gel pieces with 85% (v/v) ACN, 0.5% (v/v) TFA, lyophilised and ...
We thus tested the influence of ERAL1 deficiency on MAVS prion-like polymerization. The semidenaturing detergent agarose gel electrophoresis (SDD-AGE) assay showed that the formation of aggregated MAVS was dramatically reduced in ERAL1 knockdown HEK293T cells after SeV infection (Figure 4G). ...
The reduced transcriptional activation and promoter binding in response to MAP4K4 depletion observed in these present studies may be due to altered post- translational modifications of p65 or p50, such as acetylation that can control p65 activity and nuclear retention in a manner downstream of IkBa...
Fig. 4: Distribution of lys crotonylation and acetylation on the five human histones. Full size image Conclusions and perspectives Facts Lysine crotonylation (Kcr) is newly identified protein posttranslational modification in histone and non-histone proteins, and is involved in diverse diseases and biol...
identity of putative secreted proteins to their closest orthologs in the wheat stem rustPuccinia graminisf. sp.triticiwas dramatically reduced compared with non-secreted ones. A comparative genomics approach based on homologous gene groups unravelled positive selection in putative members of the secretome...
The last three chapters are on proteins which are the workhorses in the cell. The most fundamental property of a protein is its isoelectric point (pI) which determines what cellular components a protein can interact electrostatically (e.g., a positively
Cellular acetylation homeostasis is a kinetic balance precisely controlled by histone acetyl-transferase (HAT) and histone deacetylase (HDAC) activities. The loss of the counterbalancing function of basal HAT activity alters the precious HAT:HDAC balance