Functional motions of 15N‐labeled proteins can be monitored by solution NMR spin relaxation experiments over a broad range of timescales. These experiments however typically take of the order of several days to a week per protein. Recently, NMR chemical exchange saturation transfer (CEST) ...
Gu Y, Hansen AL, Peng Y, Bruschweiler R (2016) Rapid determi- nation of fast protein dynamics from NMR chemical exchange saturation transfer data. Angew Chem Int Ed 55:3117-3119Y. Gu, A.L. Hansen, Y. Peng, R. Bruschweiler, Rapid determination of fast protein dynamics from NMR ...
NMR chemical shifts are exquisitely sensitive to the local environment, and we show here that NMR can clearly distinguish binding (which produces a typical saturation curve) from solvent interactions (which are individually much weaker interactions and produce linear shift changes). The method is ...
Solid state NMR is a powerful method to obtain information on the structure and dynamics of protein complexes that, due to solubility and size limitations, cannot be achieved by other methods. Here, we present an approach that allows the quantification of microsecond conformational exchange in large...
Exchanging Protein Systems by Chemical Exchange SaturationTransfer.Chemicalexchange saturation transfer (CEST) NMR spectroscopy hasemerged as a powerful technique for studies of transiently formed,sparsely populated (excited) conformational states of protein moleculesin slow exchange with a dominant structure. ...
13C-labeled heparan sulfate analogue as a tool to study protein/heparan sulfate interactions by NMR spectroscopy: application to the CXCL12α chemokine J. Am. Chem. Soc., 133 (2011), pp. 9642-9645 CrossrefView in ScopusGoogle Scholar Lindahl, 2007 U. Lindahl Heparan sulfate–protein interactio...
[6,7]. These NMR approaches allow to screen and detect systems of interaction (as ligand-protein complexes) in “fast-exchange” regime, with the dissociation rate constant koffin the range 1000<koff<100 000 s−1[6,7]. Thus, ligand-based NMR approaches become useful and advantageous since...
The NMR nonequivalence of enantiomers results from the formation of transient diastereomeric solvates which may differ intrinsically in chemical shifts and which may form to different extents if the energy of interaction between homomers differs from that of enantiomers. The surroundings of "S" molecule...
chemical structure of an unknown molecule. Chemical shifts and J-couplings (internal nuclear spin interactions) are two key parameters exclusively used by chemists to predict a chemical structure. Processing samples in NMR should be diluted with deuterated solvent, such as chloroform, deuterium oxide ...
Nuclear magnetic resonance (NMR) spectra were recorded on a Varian Mercury 400 MHz spectrometer, running at 400 MHz. Chemical shifts (δ) are reported in ppm relative to the residual solvent. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were rec...