Because this directionality is an intrinsic characteristic of membrane protein structure and function, it is highly desirable to carry out structure determination within the context of bilayer lipid membranes. Solid-state NMR spectroscopy of uniaxially oriented planar bilayer lipid samples is ideally suited...
The NMR protein structure determination is driven by the optimization of collective multiple restraints arising from experimental data and the basic stereochemical properties of a protein-like molecule. By rigorous and straightforward application of CSA to the identical NMR experimental data used to ...
We present a method that measures the accuracy of NMR protein structures. It compares random coil index [RCI] against local rigidity predicted by mathematical rigidity theory, calculated from NMR structures [FIRST], using a correlation score (which assesses secondary structure), and an RMSD score ...
Solution-state NMR Membrane proteins Protein structure determination Expression systems Isotope labeling 1. Introduction Membrane proteins represent approximately 30% of proteins encoded by all genomes. They are involved in a numerous range of important cellular functions such as transport, cell-to-cell co...
Sharma, M. Zweckstetter, Simultaneous assignment and structure determination of protein backbones by using NMR dipolar couplings, Angew. Chem. Int. Ed. Engl. 43 (2004) 3479-3481.Y.S. Jung, M. Sharma, M. Zweckstetter, Simultaneous assign- ment and structure determination of protein backbones...
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy. J. Am. Chem. Soc. 130, 3959–3966 (2008). Article CAS PubMed Google Scholar Loquet, A. et al. 3D structure determination of the Crh protein from highly ambiguous solid-state NMR restraints. J. Am. Chem...
无序蛋白约占人类蛋白质组的三分之一,对调节机制和信号转导至关重要,并深刻地动摇了长期以来的结构-功能范式,但由于当时缺乏能表征蛋白质动力学性质的技术,使“有序”和“无序”两极分化,相互排斥。实际上,蛋白质的结构和动力学与功能密切相关,至关重要。现在我们逐渐认识到,蛋白质的天然状态从完全有序遍历到完...
19851990 1990 ProteinstructuredeterminationIsotopelabeling/multidimensionalNMR Pulsedfieldgradients MethodologicalMethodological Instrumental/Methodological 1995 199719982000 NMRscreening TROSYLC-NMR/LCMS-NMRCryoprobes Methodological
Determination of the NMR Structure of Gln25- Ribonuclease T[sub1]. Ribonuclease (RNase) T[sub1] is a guanyloribonuclease, having two isozymes in nature, Gln25- and Lys25-RNase T[sub1]. Between these two isozymes, there is ... Hatano,Ken-ichi,Kojima,... - 《Biological Chemistry》 被...
derive an accurate model of the structure. Once the methyl assignments have been obtained, this approach provides a rapid means to generate structural models for weakly bound protein–ligand complexes. Such weak complexes are often found at the beginning of programs of fragment based drug design ...