MLL1 and oncogenic MLL1 fusion proteins.aIllustrations of MLL1 and onco-MLL1 fusion proteins showing their functional domains. MLL1 with 3969 amino acids contains domains of (from the N- to C-terminus) Menin-binding motif (MBM, residues 2–40), LEDGF-binding domain (LBD, 109–153), AT...
p53 exists as a homotetramer, with each monomer composed of globular DNA-binding and tetramerization domains, connected by a flexible linker and flanked by intrinsically disordered regions (a transactivation domain followed by a proline-rich region at the N terminus, and a C-terminal regulatory dom...
The mycobacterial Mpa-proteasome unfolds and degrades pupylated substrates by engaging Pup’s N-terminus. EMBO J. 29, 1262–1271 (2010). Article CAS PubMed PubMed Central Google Scholar Wang, T. et al. Structural insights on the Mycobacterium tuberculosis proteasomal ATPase Mpa. Structure 17,...
Proteins representing PASTA1–2, PASTA2–4, and PASTA3–4 (domains are numbered from N to C terminus) as well as the full ECD were purified by glutathione affinity chromatography and gel filtration, and were tested for crystallization against a panel of sparse-matrix screens. Although ...
3) and GTP binding (2). The translation initiation is one of the major regulatory steps in gene expression. The direction of mRNA reading is from the 5′ end to the 3′ end, corresponding to the protein sequence from the amino (NH2or N) terminus to the carboxyl (COOH or C) terminus...
A DNA vaccine encoding the 42 kDa C-terminus of merozoite surface protein 1 of Plasmodium falciparum induces antibody, interferon-gamma and cytotoxic T cel... We have constructed a DNA plasmid vaccine encoding the C-terminal 42-kDa region of the merozoite surface protein1 (pMSP1 42) from the...
[1-556] shows 89% identity, whereas alignment between ABL1 and ABL2 C-terminal region [557-C] shows 27% identity. This suggests a regulatory mode of ABI1, distinct from its regulation of ABL1, that acts through ABL2 C-terminus to affect protein signaling downstream of actin cytoskeleton ...
N-Terminus Selective Protein Modification N末端在蛋白质的一条链中只有一处,因此以此为靶点的修饰反应必然的可以实现高位置・化学选择性,并且能够得到均质的修饰体。这样的修饰对高次结构的影响也比较小。另外大概有80%的蛋白质,其N末端都是裸露在蛋白质表面的,因此,该修饰法的活用就显得更加现实了。
蛋白纯度:N Cadherin protein purity > 70 % as determined by SDS-PAGE 标签:His & Fc Tag 蛋白构建:A DNA sequence encoding the human CDH2 (NP_001783.2) (Met 1-Ala 724) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. ...
(random picks,n = 20, i.e. ten per crosslinker dataset). Error bars show standard deviation from the mean. Source data are provided as a Source Data file.cSchematic showing error increase when merging crosslinked residue pairs to PPIs. Proteins are indicated as circles; blue and red ...