蛋白质浓度计算器(Protein Concentration Calculator)介绍:溶液中蛋白质的浓度可以通过将分子量,消光系数和λmax代入Beer-Lambert定律的派生形式来确定。 物质的λmax是其经历最强吸光度的波长。 对于蛋白质,该波长为280nm。可以使用分光光度计测量λmax处的吸光度。 注意事项: 1、蛋白质应该在溶液中很好地溶解。 蛋白...
Qubit Assay Kits use advanced Molecular Probes fluorophores that become fluorescent upon binding to DNA, RNA or protein. The selectivity of these interactions ensures more accurate results than can be obtained with UV absorbance readings; Qubit Assay ...
2. Press calculate to display the molecular weight. Data Entry Calculate Feedback Submit request References This online tool may be cited as follows MLA "QuestCalculate™Peptide and Protein Molecular Weight Calculator."AAT Bioquest, Inc.,26Feb.2025,https://www.aatbio.com/tools/calculate-peptide...
Molarity Calculator Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest....
Molarity Calculator Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest....
Qubit Assay Kits use advanced Molecular Probes fluorophores that become fluorescent upon binding to DNA, RNA or protein. The selectivity of these interactions ensures more accurate results than can be obtained with UV absorbance rea...
Protein labeling calculator (NHS activated ester chemistry)1. Molecular weight of the protein is Da Molecular weight of the protein. For IgG antibodies, MW is 150000. 2. Select label or enter label molecular weight: Da Molecular weight of the label can be obtained from the product page...
Protein concentration was determined using an A280 Nanodrop (Thermo Scientific Nanodrop lite) with the corresponding extinction coefficient (calculated based on protein sequence and using ProtParam calculator https://web.expasy.org/protparam/). 10–20 μL protein aliquots were flash-frozen using ...
Here we identify the molecular mechanism of functional selection for the disordered adenovirus early gene 1A (E1A) protein. E1A competes with host factors to bind the retinoblastoma (Rb) protein, subverting cell cycle regulation. We show that two binding motifs tethered by a hypervariable ...
(Sartorius/ForteBio). Antigen was loaded to a threshold of 1-nm shift. Tips were then washed and baselined in wells containing only Octet buffer. Samples were then associated in wells containing IgG or Fab at 100 nM concentration unless otherwise stated (other concentrations are given in ...