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This stored data file contains all relevant sample information, including time/date stamp, concentration readout, units, and assay type, as well as raw fluorescence data for assay troubleshooting. A built-in dilution calculator a...
Choose the concentration of the protein in the labeling reaction. A good starting point is 10 mg/mL. Higher concentrations lead to more efficient use of the label, but some proteins are not sufficiently soluble to get concentrated solution. Protein labeling protocol Adjust the calculator settings...
Protein concentration was determined using an A280 Nanodrop (Thermo Scientific Nanodrop lite) with the corresponding extinction coefficient (calculated based on protein sequence and using ProtParam calculator https://web.expasy.org/protparam/). 10–20 μL protein aliquots were flash-frozen using ...
B. Predicting the effect of disordered linkers on effective concentrations and avidity with the “Ceff calculator” app. Methods Enzymol. 647, 145–171 (2021). Article CAS PubMed Google Scholar Tokuriki, N., Oldfield, C. J., Uversky, V. N., Berezovsky, I. N. & Tawfik, D. S. Do...
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Concentration Volume Molecular Weight CalculateReset In Vivo Formulation Calculator (Clear solution) Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method: For example, your dosage ...
Concentration Volume Molecular Weight CalculateReset In Vivo Formulation Calculator (Clear solution) Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method: For example, your dosage ...
concentration of POI was measured in using Nanodrop (DeNovix) and POI activity was determined as described. Using POI activity and concentration values, the specific activity was calculated. tES–F116H shells containingα-andλ-conotoxins were subjected to cage break in 25 mM Tris-citrate, 0....