c, The centroid locations of peroxisomes of interest are localized using a particle localization analysis (dashed circle provided as guide for the eye). d, The GFP and mCherry intensities of each of the surrounding 17x17 pixels of the peroxisome centroid location are extracted. The intensities ...
The biosynthesis and intracellular localization of nonspecific lipid transfer protein (nsLTP) in control human subjects and in patients with peroxisome-def... Y Suzuki,S Yamaguchi,T Orii,... - 《Cell Structure & Function》 被引量: 178发表: 1990年 A novel peroxisomal nonspecific lipid‐transfer ...
The C terminus of hGSTK1 was essential for localization of the protein to peroxisomes, and the C-terminal sequence Ala-Arg-Leu represents a peroxisome targeting signal. This is the first time that a human GST has been found in peroxisomes, suggesting a new function for this family of enzymes...
PeroxisomeImmunocytochemical localizationImmunoblottingRat liverAn antibody against the non-specific lipid transfer protein from rat liver was purified by immunoabsorbent affinity chromatography. This antibody in conjunction with protein A-colloidal gold was used to localize the transfer protein in rat liver ...
基因Chapter 10 Protein Localization.pdf,Protein Localization CHAPTER OUTLINE IDI Introduction IDD The Hsp70 Family Is Ubiquitous lIB Passage Across a Membrane Requires a Special Apparatus • Members of the Hsp70 family are found in the cytosol, in the
Peroxisomal enzymes are synthesized in the cytoplasm and imported post-translationally across the peroxisome membrane. Unlike other organelles with a sealed membrane, peroxisomes can import folded enzymes, and they seem to lack intraperoxisomal chaperones. Here, we propose a mechanistic model for the ear...
Peroxisome Targeting Signal 1Objective: The aim of this study was to clone peroxisomal protein (PEP) cDNA in a mammalianexpression vector in a chimeric cDNA type, with enhanced green fluorescent protein(EGFP) cDNA. To investigate the intracellular localization of PEP protein linked to EGFPmarker, ...
CYTOSOLIC LOCALIZATION OF MOUSE PEROXISOMAL PROTEIN/ΔSKI FUSED WITH ENHANCED GREEN FLUORESCENT PROTEIN INTO CHINESE HAMSTER OVARY-K1 AND P19 CELLSPeroxisomal ProteinPeroxisome TargetingSignal PeptideObjective: Amino acid alignment analysis of deduced amino acid residues revealed a tripeptide(SKI) at the ...
Here we present an integrative framework to simultaneously interrogate the dynamics of the transcriptome and proteome at subcellular resolution by combining two methods: localization of RNA (LoRNA) and a streamlined density-based localization of proteins by isotope tagging (dLOPIT) to map RNA and ...
Cells that are not complemented by functional PEX19 show a cytosolic mislocalization of the peroxisomal marker protein eGFP-PTS1 and a diffuse background immunolabelling of the PMP PEX14, which is distinct from the peroxisomal pattern and known to reflect mislocalization to mitochondria (Fig. 5c,...