A protein “scaffold,” comparable to the antibody constant region, which fulfils the requirements for small size and high stability in an intracellular environment and carries variable regions with a high tolerance for mutation would be very valuable in this regard. Our work has demonstrated that ...
The human enzyme p97 regulates various cellular pathways by unfolding hundreds of protein substrates in an ATP-dependent manner, making it an essential component of protein homeostasis and an impactful pharmacological target. The hexameric complex underg
The cancer microbiota was considered the main risk factor for cancer progression. We had proved that Fusobacterium periodonticum (F.p) was higher abundance in Esophageal cancer(EC)tissues. Bioinformation analysis found that BCT was a key virulence protei
(GFP) was expressed inS. pombe. The GFP was used as an affinity tag and cleaved during purification (Supplementary Fig.2a). The MBP was mobile and invisible in cryo-EM. The protein fusing strategy has been successfully applied to determine the cryo-EM structures of two complexes formed ...
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coli before IPTG-induced expression; lane 2, total cellular proteins of E. coli after IPTG-induced expression at 37 for 4 h; lane 3, protein bands were visualized by Western blotting using anti-VP1 antibody; lane 4, purified cyclic VP1 (C-VP1); lane 5, purified linear VP1 (L-VP1)....
MGLAP; GIG36; Cell growth-inhibiting gene 36 protein Metabolic pathway Hematology Bone metabolism Product No.PAB477Bo01 Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species. SourcePolyclonal antibody preparation HostRabbit Potencyn/a Ig Type IgG PurificationAntigen-specific ...
Purification of the 26 kDa CAR Antigen ROS from 150 bovine retinas was prepared as described previously (34) and finally resuspended in 20 ml of 50 mM Hepes buffer, pH 7.5, containing 1.0 mM EDTA and 100 mM NaCl. The suspension was homogenized with a glass-glass tissue homogenizer and cent...
2 (TK-) cells (ATCC # CRL1764), in the presence of 5-bromodeoxyuridine (BUdR). Recombinant viruses were selected as TK-, βGal+lysis plaques. They were further subjected to three cycles of plaque purification on RAT-2 cells, again using the double TK-, βGal+selection. The purified ...
2. The antibody binding to an epitope of a complex formed by human GARP and TGF-β according to claim 1, said epitope comprising at least one of the residues 137, 138, or 139 of GARP (SEQ ID NO: 1) and at least one residue from the Latency associated peptide (LAP) of TGF-β (S...